Cell lines are useful tools to facilitate in vitro studies of many biological and molecular processes. We describe a new permanent fibroblast-type cell line obtained from disaggregated Xenopus tropicalis limb bud. The cell line population doubling time was ~24 h. Its karyotype was genetically stable with a chromosome number of 2n = 21 and a chromosome 10 trisomy. These cells could be readily tr...

Aegilops variabilis (UUSvSv), an important sources for wheat improvement, originated from chromosome doubling of a natural hybrid between Ae. umbellulata (UU) with Ae. longissima (SlSl). The Ae. variabilis karyotype was poorly characterized by fluorescent in situ hybridization (FISH). The FISH probe combination of pSc119.2, pTa71 and pTa-713 identified each of the 14 pairs of Ae. variabilis chr...

We have proposed a hypothetical model to explain the role of chromosomal aberrations in malignant development. In this model we postulate two kinds of chromosomal changes: (1) primary, active changes caused by direct inter action between the oncogenic agent and the hereditary material of the host cello These changes are mainly somatic mutations, but mayaIso be associated with directed structura...

Two epithelial cell lines were established from separate pools of cells that were harvested by a sequential proteolytic treatment of a single specimen of rat transplantable colonic carcinoma. The cells were small (10-micrometer diameter) and cuboidal and displayed desmosomes and light junctions. Both cell lines grew rapidly with population-doubling times of 20 to 22 hr, were near diploid in chr...

BACKGROUND AND OBJECTIVE Lung cancer is a major worldwide health problem. The aim of this study is to establish a novel Chinese human lung adenocarcinoma cell line and examine its biological characteristics. METHODS Lung adenocarcinoma specimens were freshly resected during surgery. The tissues were incubated in vitro and the cell line was named Ch-Huang-1. The biological characteristics of t...

Hybrids (2n = 3x = 21) between Aegilops ovata and Secale cereale were produced via embryo rescue. Most hybrid morphological traits were intremediate between parents, the plants grew vigorously but were completely sterile. The average frequency of chromosome associations at metaphase I was 19.57-20.19 univalents and 0.40-0.86 rod bivalents. The fertility of the hybrids was restored by doubling t...

Miscanthus × giganteus is a popular industrial plant with great potential in ecological agriculture. It forms numerous rhizomes that are important the sequestration of carbon dioxide. The can be source lignin and cellulose, biomass for renewable energy production, used small garden architecture, or to strengthen banks landslides. Breeding this species difficult, as it sterile allotriploid 57 ch...