We report here a novel method to simultaneously detect CpG methylation and single nucleotide polymorphisms (SNPs) using denaturing high performance liquid chromatography (DHPLC). PCR products of bisulfite-modified CpG islands were separated using DHPLC. BstUI digestion and DNA sequencing were used in confirmation studies. Consistent with the BstUI digestion assay, the 294 bp PCR product of the ...

n Thin-layer chromatography (TLC) is being used increasingly for the separation of the lipid classes (1, 2) prior to the analysis of their fatty acids by gas-liquid chromatography (GLC). Malins and Mangold (3) have described the separation of the methyl esters of fatty acids of menhaden oil from other lipid constituents and stated “the gas chromatogram of the total methyl ester fraction agreed ...

The enzyme involved in hexenuronic acid (HexA) removal from kraft pulp was identified in Paenibacillus sp. strain 07. Extracellular and intracellular enzymes of Paenibacillus sp. were assessed for their hexenuronosylxylotriose (∆X3) degradation activity. First, ∆X3 was obtained from hardwood kraft pulp by enzymatic hydrolysis using three commercial enzymes. Crude extracellular and intracellular...

Single radial immunodiffusion (SRID) assay requires a reference antigen and an antibody to the hemagglutinin (HA) of an influenza vaccine. As it takes 2‑3 months to develop the reference antigen, vaccine development is delayed in cases of an influenza pandemic. In the present study, the measurement of the HA content of influenza vaccines was assessed using size exclusion high performance liquid...

In the biological sciences, mass spectrometry is one of the most popular methods employed for the determination of the molecular weights and structures of metabolites. The popularity of mass spectrometry is due to the high sensitivity of the analytical method and the possibility of obtaining molecular weights and structure of metabolites directly from high performance liquid chromatography (HPL...

Ethylene gas is a naturally occurring gas that has an influence on the shelf life of fruit during their transportation in cargo ships. An unintentional exposure of ethylene gas during transportation results in a loss of fruit. A gas chromatographic system is presented here for the detection of ethylene gas. The gas chromatographic system was assembled using a preconcentrator, a printed 3D print...

The determination of the molecular weight distribution by gel permeation chromatography requires the establishment of a molecular weight calibration and efficient columns in order to minimise chromatogram broadening. The separation process is described in terms of steric exclu— sion, retardation and partial exclusion mechanisms in order to show how the universal calibration method may be influe...

As columns age and differ between systems, retention times for comprehensive two-dimensional gas chromatography (GCxGC) may vary between runs. To properly analyze GCxGC chromatograms, it often is desirable to align the retention times of chromatographic features, such as analyte peaks, between chromatograms. Previous work by the authors has shown that global, low-degree polynomial transformatio...

Primycin is a macrolide antibiotic complex produced in microbiological fermentation processes. The microbial production of primycin requires an in-process analytical method suitable for monitoring the level of the active agents. In this paper, a method fulfilling the described requirement is presented. This method consists of a simple, efficacious extraction step, an instrumental sample applica...

When volatile solvents are used for paper chromatography, as in the systems described by Bush (1952) for separation of steroids, it is usually necessary to hang the paper in the tank for several hours' equilibration before the addition of the mobile phase. If equilibration is allowed to proceed overnight much of the next day will be taken up with running the chromatogram. An apparatus has there...