OBJECTIVE To delineate the significance of maternal uniparental disomy 14 (upd(14)mat) and related disorders in patients with a Prader-Willi syndrome (PWS)-like phenotype. STUDY DESIGN We examined 78 patients with PWS-like phenotype who lacked molecular defects for PWS. The MEG3 methylation test followed by microsatellite polymorphism analysis of chromosome 14 was performed to detect upd(14)m...

Both MAT-B1 (nontransplantable) and MAT-Cl (xenotransplantable) ascites sublines of the 13762 rat mammary adenocarcinoma contain a major sialoglycoprotein (ASGP-1) with low electrophoretic mobility in dodecyl sulfate. ASGP-1 from each subline is rapidly purified by density gradient centrifugation of membrane fragments in cesium chloride containing 4 M guanidine hydrochloride. The products give ...

Mating-type genes control the entry into the sexual cycle, mating identity and sexual development in fungi. The mat A-2 and mat A-3 genes, present in the mat A idiomorph of the filamentous fungus Neurospora crassa, are required for post-fertilization functions but are not essential for mating identity. Their putative roles as transcription factors are based on the similarity of mat A-2 with the...

BACKGROUND Leptospirosis is diagnosed on clinical grounds, and confirmed by microscopic agglutination test (MAT). IgM-ELISA (Serion-Virion) and immunochromatography test (Leptocheck-WB) are two immunodiagnostic assays for leptospirosis. Their sensitivity, specificity and applicability in Sri Lanka have not been systematically evaluated. METHODS Clinically diagnosed leptospirosis patients (n =...

Utilization and management of Empty Fruit Bunches (EFB) fibre continue to develop as the main ingredient additional material used in various industrial products. The technological breakthrough targeted this study is developed EFB a filler polymer composite partition boards which are heat retainers interior construction buildings. board insulator, its thermal conductivity affected by mass densit...

In the heterothallic filamentous fungus Podospora anserina, four mating-type genes encoding transcriptional factors have been characterized: FPR1 in the mat+ sequence and FMR1, SMR1, and SMR2 in the alternative mat- sequence. Fertilization is controlled by FPR1 and FMR1. After fertilization, male and female nuclei, which have divided in the same cell, form mat+/mat- pairs during migration into ...

The fungal pathogen Ustilago hordei causes the covered smut disease of barley and oats. Mating and pathogenicity in this fungus are controlled by the MAT locus, which contains two distinct gene complexes, a and b. In this study, we tagged the a and b regions with the recognition sequence for the restriction enzyme I-SceI and determined that the distance between the complexes is 500 kb in a MAT-...

The fungal genus Stemphylium (Ascomycota) contains selfing species that evolved from outcrossing ancestors. To find out how selfing originated, we analyzed the Stemphylium MAT loci that regulate sexual reproduction in ascomycetes and compared MAT structures and phylogeny with a multigene Stemphylium species phylogeny. We found that some Stemphylium species' MAT loci contained a single gene, eit...