OBJECTIVE To assess the clinical effectiveness and cost-effectiveness of glucosamine sulphate/hydrochloride and chondroitin sulphate in modifying the progression of osteoarthritis (OA) of the knee. DATA SOURCES Electronic databases were searched from 1950 to 2008 and included: MEDLINE and PubMed; EMBASE; Cochrane Library (including Cochrane Systematic Reviews Database, CENTRAL, DARE, NHS EED ...

Newly synthesized rat glomerular [35S]proteoglycans were labelled in vivo after injecting Na2[35S]SO4 intraperitoneally. At the end of the labelling period (7 h) the kidneys were perfused in situ with 0.01% (w/v) cetylpyridinium chloride. This fixed proteoglycans in the tissue and increased their recovery 2-3-fold during subsequent isolation of glomeruli from the renal cortex. The glomeruli wer...

Proteus vulgart8 yields N-acetylchondrosin. On the bases of these findings, together with analytical results, the product is considered to be N-acetylchondrosin sulphate. 3. When chondroitinase action is notallowed to proceed to completion a series of oligosaccharides is obtained, the principal member being identical with the single product obtained by exhaustive degradation. This series of oli...

Previous studies have strongly suggested an association between glycosaminoglycans and tissue deposits of amyloid. The present study was aimed at studying this association in purified preparations of hepatic amyloid fibrils obtained from human AA type secondary amyloidosis. Glycosaminoglycans were isolated by gradient ion exchange chromatography of purified amyloid fibrils treated with pronase....

Native collagen, acetylated collagen, collagen/10% chondroitin sulphate, collagen/2.5% hyaluronic acid and collagen/20% hyaluronic acid were implanted both as film and as sponge into rat lumbar muscle for 7 and 14 d. After 7 d implantation, all materials elicited an acute inflammatory cell response characterized by numerous polymorphs and histocytes. The cell population after 14 d was principal...

HCI in lOml of methanol and diluting to IOOml with butan-1-o1;just before use, 0.1 g of sodium dithionite was added. The dried electrophoresis sheets were sprayed with the reagent and developed at 120°C for 1 5min. With 4and 6-sulphated disaccharides a strong colour-positive reaction occurred with p-anisidine at the highest amount examined (20pg) and a detectable coloured product was observed a...

OBJECTIVES To analyse the glycosaminoglycans of the adult human rotator cuff tendon matrix, to characterise changes in the glycosaminoglycan composition with age and in chronic rotator cuff tendinitis. METHODS Rotator cuff (supraspinatus) tendons (n = 84) and common biceps tendons (n = 26) were obtained from cadavers with no history of tendon pathology (age range 11-95 years). Biopsies of rot...

Carbohydrase and sulphata-se activities of Porphyra umbilicalis. By S. PEAT and D. A. REEs 7 Equilibrium constants of phosphoryl transfer from CM1 to C(6) Of Oa-D-glucose 1-phosphate and from glucose 6-phosphate to water. By M. R. ATKiNsoN, E. JoHNsoN and R. K. MoRTow 12 -The chemistry of connective tissues. 6. The constitution of the chondroitin sulphate-protein complex in cartilage. By S. M. ...