Choline dehydrogenase is the initial enzyme of the complete choline oxidase system. The latter includes all agents and enzymes involved in hydrogen transport to oxygen plus the initial dehydrogenase. Problems concerning the properties of choline dehydrogenase have remained unsolved largely because of the insoluble nature of the enzyme. It has resisted solution by such techniques as freezing and...

Serum cholinesterase activity and the dibucaine numbers have been determined by using a hydrogen peroxide electrode and the enzyme choline oxidase immobilized on a nylon net. The analysis procedure is extremely simple and very fast allowing 30 cholinesterase determinations per hour. Both cholinesterase activity and dibucaine number measurements could be performed in 5 min and by using serum sam...

Carvol and carvone are oxidation products from the natural product limonene. They important raw materials for flavours fragrances industry also act as pharmaceutical active ingredients. Orange waste peels possibly represent an attractive source limonene, but studies on valorizing orange peel wastes rare. In this study, we report a new enzymatic cascade system in-situ conversion of limonene into...

Well defined patterns of quantitative distribution of carbonic anhydrase have been reported in the central nervous system of man and of animals (1,2). Two reports have been made on deviations from the normal pattern of distribution in man incident to mental disease (3,4). As part of an effort to elucidate the significance of this enzyme in the central nervous system, a study of its fetal distri...

Background: Many in vitro studies suggested that choline incorporation into many types of tumors is related to cell proliferation. Whether in vivo choline incorporation is related also to cell proliferation or not was my question. Therefore, the aim of this pilot study was to investigate the relationship of in vivo [methyl- 14C]-choline incorporation and in vivo [methyl-3H]- thymidine incorpora...

During neuronal communication neurotransmitters are released through the process exocytosis that occurs on the time scale of sub-milliseconds to milliseconds. Neuronal activity by neurotransmitters that are non-electroactive cannot easily be monitored due to limitations in the temporal resolutions of the sensor probes for these analytes. In order to achieve a fast detection of the rapid transie...

Abstract Background Fungal glucose dehydrogenases (GDHs) are FAD-dependent enzymes belonging to the glucose-methanol-choline oxidoreductase superfamily. These classified in “Auxiliary Activity” family 3 (AA3) of Carbohydrate-Active enZymes database, and more specifically subfamily AA3_2, that also includes closely related flavoenzymes aryl-alcohol oxidase 1-oxidase. Based on sequence similarity...