نتایج جستجو برای: cholesterol oxidase
تعداد نتایج: 135665 فیلتر نتایج به سال:
Methyl sterol oxidase of microsomal synthesis of cholesterol from lanosterol is a mixed-function oxidase that is dependent upon reduced pyridine nucleotide. The methyl sterol oxidase, as well as NADH-cytochrome c reductase, in intact rat liver microsomes are inhibited by anti-cytochrome b5 immunoglobulin, but NADPH-cytochrome c reductase is not affected. There is a decreased time lag prior to o...
A sensitive and selective chemiluminescence (CL) sensor based on the peroxidase-like activity of copper nanoclusters was established for the detection of cholesterol. Copper nanoclusters catalyse the CL reaction between luminol and H2O2. Because H2O2 is the oxidative product of cholesterol in the presence of cholesterol oxidase, the oxidation of cholesterol can be quantitatively converted to a ...
We have developed an automated method for measuring high-density lipoprotein (HDL)-cholesterol in serum without prior separation, using polyethylene glycol (PEG)-modified enzymes and sulfated alpha-cyclodextrin. When cholesterol esterase and cholesterol oxidase enzymes were modified with PEG, they showed selective catalytic activities towards lipoprotein fractions, with the reactivity increasin...
The present study describes the effect of hydrolysis polyethyleneimines in water/ethanol mixture on morphology cobalt oxide (Co3O4), used as main sensor component. structure generated Co3O4 nanocrystals is consistent with a well-defined cubic phase crystallography, having only and oxygen elements. Developing simple, low-cost, sensitive, selective cholesterol biosensors essential for accurate mo...
Diet-induced hypercholesterolemia leads to oxidative/nitrative stress and subsequent myocardial dysfunction. However, the regulatory role of microRNAs in this phenomenon is unknown. We aimed to investigate, whether hypercholesterolemia-induced myocardial microRNA alterations play a role in the development of oxidative/nitrative stress and in subsequent cardiac dysfunction. Male Wistar rats were...
In this study, use of the covalent enzyme immobilization method was proposed to attach cholesterol oxidase (ChO) on a conducting polymer, poly(3-thiopheneacetic acid), [poly(3-TPAA)]. Three red-orange poly(3-TPAA) films, named electrodes A, B and C, were electropolymerized on a platinum electrode by applying a constant current of 1.5 mA, for 5, 20 and 100 s, respectively. Further, 1-ethyl-3-(3-...
A sensitive fixed-time kinetic enzymatic method for the measurement of high density lipoprotein cholesterol is described, as adapted for the Cobas Bio centrifugal analyser (Hoffmann LaRoche). The Boehringer Mannheim cholesterol esterase/cholesterol oxidase/peroxidase/3,4-dichlorophenol kinetic reagent was modified by the inclusion of 2,4,6-tribromo-3-hydroxybenzoic acid which reacts with hydrog...
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