One concern about using transgenic plants is the genetic variation that occurred from theirs tissue culture and regeneration. Molecular markers are an important element for efficient and effective determination of genetic variation. The present work was carried out to assess the genetic uniformity of transgenic cottons (Bt and chitinase lines), using RAPD, ISSR molecular markers and SDS-PAGE an...

In the present investigation, 34 Bacillus thuringiensis (Bt) isolates of different agroclimatic condition were studied for fungitoxic characters and the principle involved for fungitoxicity was identified in most of the cases as exochitinases. The different Bt strains were screened for fungitoxicity and chitin degrading activity employing 4 fungal pathogens. Biochemical assay of the chitinase u...

We have used differential display to identify genes that are regulated by juvenile hormone in the epidermis of the beetle Tenebrio molitor. One of the genes encodes T. molitor chitinase 5 (TmChit5), a chitinase possessing an unusual structure. Sequence analysis of TmChit5 identified five 'chitinase units' of approx. 480 amino acids with similarity to chitinase family 18. These units are separat...

We cloned a complete cDNA encoding rye seed chitinase-c, designated RSC-c, by rapid amplification of cDNA end and PCR procedures. The cDNA of RSC-c consists of 1,018 nucleotides and includes an open reading frame encoding a polypeptide of 266 amino acid residues. A recombinant RSC-c was produced by expression in Escherichia coli Origami(DE3) and purified. rRSC-c had almost the same chitinase ac...

Disruption of genes in medically important fungi has proved to be a powerful tool for evaluation of putative virulence factors and identification of potential protein targets for novel antifungal drugs. Chitinase has been suggested to play a pivotal role in autolysis of the parasitic cell wall of Coccidioides immitis during the asexual reproductive cycle (endosporulation) of this systemic patho...

Abstract Food crop security and is a challenge in the agricultural sector to produce quality healthy food products for consumption by community. The use of synthetic fungicides induce plant resistance negatively affects abundance diversity biological agents.Cellulose enzymes chitinase are capable lytic that degrade cell walls pathogenic fungi plants. This study aims isolate organic rice rhizosp...

In this paper, we describe the structure of chitinase B from Serratia marcescens, which consists of a catalytic domain with a TIM-barrel fold and a 49-residue C-terminal chitin-binding domain. This chitinase is the first structure of a bacterial exochitinase, and it represents one of only a few examples of a glycosyl hydrolase structure having interacting catalytic and substrate-binding domains...

Analysis of clones isolated from a cosmid DNA library indicates that the Serratia marcescens chromosome contains at least two genes, chiA and chiB, which encode distinct secreted forms of the enzyme chitinase. These genes have been characterized by inspection of chitinase activity and secreted proteins in Escherichia coli strains containing subclones of these cosmids. The two chitinase genes sh...

Investigations on the development of a biocontrol agent against the alarming stored food pest Corcyra cephalonica has witnessed noteworthy growth in current years from mainly physiological studies, to tackling fundamental queries concerning the underlying mode of action. Chitinase from Trichoderma viride, which has inauspicious effects on feeding behavior, development and biochemical parameters...

Sorghum serves as staple food for millions of people in Asia. It ranks as the sixth most planted crop and it is vulnerable to fungal diseases resulting in decreased grain quality and yield loss. Agrobacterium strains used were LBA4404 harbouring pcambia-ubi-chi11 (rice chitinase), EHA105 harbouring pcambia-ubi RC7 (rice chitinase) with bar gene and EHA105 harbouring pMKURF2 (rice chitinase gene...