A design for a chemotaxis chamber and its use in bacterial chemotaxis experiments are described. Some of the advantages of the new design are discussed.

Microfluidics is revolutionizing the way we study the motile behavior of cells, by enabling observations at high spatial and temporal resolution in carefully controlled microenvironments. An important class of such behavior is bacterial chemotaxis, which plays a fundamental role in a broad range of processes, including disease pathogenesis, biofilm formation, bioremediation, and even carbon cyc...

Chlamydomonas reinhardtii expresses a well-documented circadian rhythm of phototaxis, which peaks in the subjective daytime. We find that vegetative cells also express circadian rhythms of chemotaxis to ammonium and ammonium uptake (as gauged by uptake of [(14)C]methylammonium). The chemotaxis rhythm peaks in the subjective night. Methylammonium uptake is light dependent, and its rhythm peaks a...

Chemotaxis, a directional cell migration guided by extracellular chemoattractant gradients, plays an essential role in the recruitment of neutrophils to sites of inflammation. Chemotaxis is mediated by the G protein-coupled receptor (GPCR) signaling pathway. Extracellular stimuli trigger activation of the PLC/PKC/PKD signaling axis, which controls several signaling pathways. Here, we concentrat...

In this paper we introduce a bacteria-leukocyte model with bacteria chemotaxsis. We assume that bacteria develop a tactic defence mechanism as a response to Leukocyte phagocytosis. We explore the effect of this tactic motion on Turing space in two parameter spaces. A fine tuning of bacterial chemotaxis shows a significant effect on developing a non-uniform steady state. Keywords—Chemotaxis-diff...

Chemotaxis is a type of cell movement in response to chemical stimulus which plays key role multiple biophysical processes, such as embryogenesis and wound healing, crucial for understanding metastasis cancer research. In the literature, chemotaxis has been modelled using models based on systems nonlinear stochastic partial differential equations (NSPDEs), are known be challenging statistical i...

Microfluidics confers unique advantages in microbiological studies as these devices can accurately replicate the micro- and even nano-scale structures of soil to simulate habitats bacteria. It not only helps us understand spatial distribution bacterial communities (such biofilms), but also provides mechanistic insights into microbial behaviors including chemotaxis horizontal gene transfer (HGT)...

Neutrophil recruitment and directional movement toward chemotactic stimuli are important processes in innate immune responses. This study examines the role of Fer kinase in neutrophil recruitment and chemotaxis to various chemoattractants in vitro and in vivo. Mice targeted with a kinase-inactivating mutation (Fer(DR/DR)) or wild type (WT) were studied using time-lapse intravital microscopy to ...

Existing chemotaxis assays do not generate stable chemotactic gradients and thus--over time--functionally measure only nonspecific random motion (chemokinesis). In comparison, microfluidic technology has the capacity to generate a tightly controlled microenvironment that can be stably maintained for extended periods of time and is, therefore, amenable to adaptation for assaying chemotaxis. We d...

Chemotaxis, the orientedmovement of cells in response to ambient chemical gradients, is a prominent feature in the organization ofmany biological populations. Since the pioneer work of Keller and Segel [11] to propose mathematical models for chemotaxis, there has been great interest in modeling chemotaxis and in the mathematical analysis of systems like the Keller-Segel model. In this paper, mo...