Peptides containing phosphoserine or phosphothreonine were synthesized by solid phase methods. Phosphoserine and phosphothreonine were incorporated into peptides using Boc-diphenylphosphono esters of serine and threonine and standard DCC/HOBt coupling. The phenylphosphoesters were not removed when the peptides were cleaved from the resin by HF or by trifluoromethane sulfonic acid, but were subs...

A non-destructive method of collecting samples for DNA analysis of the Chinese giant salamander is described and validated. DNA was extracted from the skin secretion and shedding using a Chelex-based method, and partial 12S rRNA gene sequences were amplified and sequenced. Sequences from skin secretion and shedding were cross-checked against the reported sequences from liver and were found to b...

Chelates can inhibit the iron- and copper-catalyzed autoxidation of ascorbate at pH 7.0. Diethylenetriaminepentaacetic acid (DTPA or DETAPAC) and Desferal (deferoximane mesylate) slow the iron-catalyzed oxidation of ascorbate as effectively as reducing the trace levels of contaminating iron in buffers with Chelex resin. DETAPAC, EDTA and HEDTA (N-(2-hydroxyethyl)-ethylenediaminetriacetic acid) ...

fasciolosis is an important parasitic disease common among humans and livestock. it is considered as a health problem and causes great economic losses in several regions of the world. considering the prevalence of fascioliasis, study on molecular aspect of the parasite is so much important. the present study was carried out to achieve a suitable method of dna extraction from fasciola for furthe...

The spin-polarized calculations in generalized gradient approximation density–functional theory (GGA–DFT) have been used to show how the existence of second metals can modify the atomic hydrogen adsorption on Au (100), Cu (100), and Pt (100) surfaces. The computed adsorption energies for the atomic hydrogen adsorbed at the surface coverage of 0.125 ML (monolayer) for the monometallic Au (100), ...

Polymerase chain reaction (PCR)based assays have been developed to identify microscopic organisms that cause disease (8) or have an environmental impact (1). In both cases, DNA is extracted from pooled individuals. The pooled sample has typically been created for bacteria by culturing and for disease vectors by taking advantage of their reproduction in the host. PCRbased assays are being develo...

This paper deals briefly with a technique developed for the identification of the early stages of veligers of the surfclam Spisula solidissima from the larval stages of other common bivalve species using a combination of DNA probe and restriction fragment length polymorphism (RFLP) analysis. An oli¬ gonucleotide sequence designed from the 18S ribosomal RNA gene (nucleotide position 259-276) pro...

AIM To determine allele frequencies' distribution for 15 short tandem repeat (STR) loci in a population sample of 1910 unrelated individuals from the region of Wallachia, South Romania. METHODS DNA was isolated using Chelex 100 method and an adapted version of AGOWA mag DNA Isolation Kit Sputum. Polymerase chain reaction amplification was done using AmpFlSTR Identifiler kit (D8S1179, D21S11, ...

The ascorbate oxidase activity of ceruloplasmin has been a subject of controversy because of (a) the relatively low oxidase activity of this serum copper protein toward ascorbic acid and (b) the different conclusions reached by several investigators. In 1951, Holmberg and Laurel1 (1) reported that ascorbic acid was a substrate of ceruloplasmin although it was less active as a substrate than p-p...

Boiled cytosols prepared from a wide variety of sources contain a low Mr factor that inhibits glucocorticoid receptor transformation to the DNA-binding state (Leach, K.L., Grippo, J.F., Housley, P.R., Dahmer, M.K., Salive, M.E., and Pratt, W.B. (1982) J. Biol. Chem. 257, 381-388). In this work, we show that this endogenous factor, which is partially purified from rat liver, produces all of the ...