Disposable reversed-phase chromatography columns were tested for their effectiveness in removing unreacted trichloroethanol (TCE) from derivatized samples for gas-liquid chromatography analysis. Derivatized acidic chloroform extracts of saponified whole cells of Mycobacterium species, spent culture media, and derivatized acidic chloroform extracts of serum and cerebrospinal fluids from patients...

Research of new diagnosis or prognosis biomarkers is a major challenge for the management of patients with complex pathologies like cancer. Clinical proteomics is one of the recent approaches to identify these biomarkers in biological fluids. Over the last five years, many problems related to the variability and the quality control of these analyses have been observed. This was notably related ...

HE BIURET REACTION has become an important analytic procedure for rapid and accurate determinations of protein concentrations in biologic fluids. Since the initial reports of Riegler (1), Autenrieth and Mink (2), and Autenrieth (3), who showed that proteins react with alkaline copper solutions to yield a violet-blue color, many modifications have appeared to improve the stability of alkaline co...

1 CHEMERIN 158K IS THE DOMINANT CHEMERIN ISOFORM IN SYNOVIAL AND CEREBROSPINAL FLUIDS BUT NOT IN PLASMA. Lei Zhao, Yasuto Yamaguchi, Shadi Sharif, Xiao-Yan Du, David M. Lee, Lawrence D. Recht, William H. Robinson, Jason J. Song, John Morser and Lawrence L.K. Leung 6* From the Division of Hematology and Division of Immunology and Rheumatology, Department of Medicine, Department of Neurology and ...

The use of high-performance liquid chromatography (HPLC) to measure ribavirin in serum and other biological fluids has been limited by endogenous interfering substances. We report an HPLC procedure based on the extraction of ribavirin from serum, plasma, or cerebrospinal fluid with a boronate affinity gel, which uses a 3-methylcytidine internal standard. This assay is sensitive (to 0.4 microM),...

Spectrophotometric examinations were performed on cerebrospinal and subdural fluids in subacute (five patients) and chronic (20 patients) subdural haematomas, with special reference to the diagnostic aid of CSF spectrophotometry. Spectrophotometric xanthochromia of haemorrhagic origin was found in all CSFs examined, while definite visible xanthochromia was observed in only 28% and the CSF was j...

The concentrations of morphine and 6-acetylmorphine (6-AM) in urine, cerebrospinal fluid (CSF), and vitreous humor (VH) and the morphine concentrations in blood were determined by gas chromatography-mass spectrometry for 29 fatalities after abuse of heroin either alone or in combination with alcohol and other drugs. 6-AM was found above a quantitation limit of 1 ng/mL in urine in 89% of the cas...

Direct read-out of chloride concentration is provided by an auxiliary designed for use with the automatic coulometric-amperometric titrator of Cotiove et al. (1). The circuitry, construction, and operation of the auxiliary are described. Automatic titration of samples of biologic fluids such as serum, cerebrospinal fluid, urine, sweat, and gastrointestinal fluids requires no prior preparation a...

The microtubule-associated protein Tau is mainly expressed in neurons of the CNS and is crucial in axonal maintenance and axonal transport. The rationale for Tau as a biomarker of neurodegenerative diseases is that it is a major component of abnormal intraneuronal aggregates observed in numerous tauopathies, including Alzheimer's disease. The molecular diversity of Tau is very useful when analy...

The enumeration and identification of blood cells in body fluids offers important information for the diagnosis and treatment of various medical conditions. Manual microscopic methods (hemacytometer total cell count and cytocentrifuged differential count) have inherent analytic and economic disadvantages but are still considered the "gold standard" methods. We evaluated the analytic and clinica...