The endoglucanase cDNA, Dvv-ENGase I, from western corn rootworm, Diabrotica virgifera virgifera LeConte was expressed using the GS115 methylotrophic strain of Pichia pastoris. The Dvv-ENGase I gene was cloned into the integrative plasmid pPICZαA under the control of AOX1, which is a methanol-inducible promoter. Positive clones were selected for their ability to produce the recombinant endogluc...

The use of filter aids and precoat filtration is ubiquitous in a wide number of industries, including chemicals, food processing, pharmaceuticals, mining, municipal (potable) water treatment and waste treatment. World Minerals Inc., the parent company of Celite and Harborlite, and other organizations have recently made major advances in filter aid technology. These advances have now pushed the ...

introduction : albumin, one of the most important plasma proteins, has a difficult process of synthesis and production. we compared two different methods for albumin purification: carboxymethyl cellulose (cm cellulose) resin exchange and diethylaminoethyl cellulose (deae cellulose) resin exchange in order to determine which resin could be more beneficial. materials and methods : two ion exchang...

In this paper we explore the direct transfer via lamination of chemical vapor deposition graphene onto different flexible substrates. The transfer method investigated here is fast, simple, and does not require an intermediate transfer membrane, such as polymethylmethacrylate, which needs to be removed afterward. Various substrates of general interest in research and industry were studied in thi...

Alpha toxin derived from Clostridium perfringens was used for diagnosis of Streptococcus agalactiae (St.agalactiae) by rapid and spot CAMP tests. The selected Cl.perfringens strain was cultured in optimized conditions; the supernatant of culture was concentrated by ultra filter and purified by DEAE cellulose chromatography. The efficacy of both purified and crude α-toxins were examined by rapid...

Abstract The konjac polysaccharide aerogel filter rod has the functions of high filtration efficiency and cooling. biodegradation rate prepared by vacuum freeze drying machine was studied in this paper. results showed that at end degradation experiment (the 106 th day), gel reached 84.9%, which close to thin-layer chromatography grade cellulose, indicating it had good biodegradability.

Understanding the adsorption of smoke stream (SR) on cigarette filter—cellulose acetate stick with different temperatures is beneficial for controlling chemical emissions and reducing toxic effect smoking human health. However investigation corresponding properties was missing because substances cellulose sensitive three-dimensional temperature gradient. In this work, typical substances, such a...

Bioconversion, particularly enzymatic hydrolysis of cellulosic materials to useful products has great potential and is being actively investigated [l-31. Biotechnological utilization of cellulose requires a very efficient cellulolytic enzyme system active on native cellulose. Cellulases are nulticomponent enzyme systems in which the synergistic action of endoglucanase, exoglucanase and Betagluc...

there are many reports on the application of different hydrocolloids in the production of leavened breads; most of which show positive effects on bread quality. however, there is little information to prove such effect on flat breads. the main aim of this study was to improve the quality of barbari dough and bread (iranian flat bread) in terms of texture, taste and general acceptability of fres...

BACKGROUND Expansins are relatively small proteins that lack enzymatic activity and are found in plants and microorganisms. The function of these proteins is to disrupt the plant cell walls by interfering with the non-covalent interchain bonding of the polysaccharides. Expansins were found to be important for plant growth, but they are also expressed by various bacteria known to have interactio...