The permeability characteristics of biomaterials are critical parameters for a variety of implants. To analyse the permeability of membranes made from crosslinked ultrathin gelatin membranes and the transmigration of cells across the membranes, we combined three technical approaches: (1) a two-chamber-based permeability assay, (2) cell culturing with cytochemical analysis and (3) biochemical en...

Giant polymersomes are versatile and stable biomimetic compartments that ideal for building cell-like systems. However, the transport of hydrophilic molecules across membrane, which controls function systems, is limited by low permeability polymeric bilayers. Therefore, mechanisms to control necessary create functional Here, we describe design giant equipped with spiropyran-based modulators. Ph...

We aimed to develop a high-throughput screening (HTS) system for preliminary predictions of human skin permeability by using an artificial membrane that can mimic the permeation behaviour of lipophilic and hydrophilic compounds across the human skin. In this study, we synthesized a copolymer containing poly(dimethylsiloxane) (PDMS) and poly(ethylene glycol) (PEG) 6000 and impregnated it onto a ...

Non-enveloped viruses must deliver their viral genome across a cell membrane without the advantage of membrane fusion. The mechanisms used to achieve this remain poorly understood. Human rhinovirus, a frequent cause of the common cold, is a non-enveloped virus of the picornavirus family, which includes other significant pathogens such as poliovirus and foot-and-mouth disease virus. During picor...

Two different sizes of siRNAs, of which one type was 21-nucleotide (nt) siRNA containing 2-nt dangling ends and the other type was 27-nt siRNA with blunt ends, were conjugated with a nuclear export signal peptide of HIV-1 Rev at the 5'-sense end. Processing by Dicer enzyme, cell membrane permeability, and RNAi efficiency of the peptide-conjugated siRNAs were examined. Dicer cleaved the peptide-...

Metal-organic-framework nanoparticles (MOF NPs) have been increasingly used to encapsulate therapeutic enzymes for delivery. To better interface these MOF NPs with biological systems, researchers coated them natural cell membranes, enabling biomimicking properties suitable innovative biomedical applications. Herein, we report that the enzymatic activity of cell-membrane-coated can be significan...

Direct ethanol fuel cells (DEFCs) offer a high degree of design flexibility, ranging from single cell to massive multi-cell that can be used in various applications, including portable devices, transportation, and stationary applications. Unfortunately, the most significant barrier commercialization DEFCs is getting low-cost permeability, conductivity performance, extended durability polymer el...

Chitosan/polyethersulfone (CS/PES) composite membrane was prepared from casting chitosan solution on polyethersulfone substrate membrane. The Substrate membrane was prepared by phase inversion technique using polyethersulfone (PES) and dimethylacetamide (DMAc) as solvent with and without the addition of polyvinylpyrrolidone (PVP) as pore-forming agent. The effects of the composition of the c...