the immounological responses to s. aureus cell wall antigens, namely crude or whole cell, teichoic acid (ta) peptidoglycan prepared in conventional way (cpg), cell wall (cw), lipoteichoic acid (lta) were investigated by number of immunological methods e.g. ria, elisa, immunodiffusion and western blotting techniques.also a method for preparation of peptidoglycan (mpg) was modified and used as va...

ELISA data is usually handled in the MS Excel worksheets through different methods and different formulas. Accurate and precise calculations of the data is time consuming and tedious. ELISA Analyzer was developed with a user friendly interface to streamline titration and analysis of the data obtained from ELISA and improve rapid and lucid presentation of the results. The software can also hel...

Introduction. The ability of SARS-CoV-2 antibodies to neutralize the virus is primary indicator their specific activity. test for neutralizing (NAbs) much needed in different biomedical studies.The aim study find optimum conditions microscopic and spectrophotometric detection SARSCoV-2 NAbs by inhibition cytopathic effect (CPE) cell cultures.Materials methods. Blood sera collected from COVID-19...

background: tuberculosis (tb) remains an important health problem throughout the world. despite its significance in public health, mechanisms of protective immunity against mycobacyerium tuberculosis in humans have not yet been understood. objective: to evaluate cell mediated immune response against purified ag 85, ppd and phytohemagglutinin (pha) in patients with tuberculosis and healthy tuber...

introduction: the aim of this study was to determine a proper cell line for adherance of helicobacter pylori and a suitable method for attachment of this bacteria to cell lines in vitro. material and methods: a total of 22 h.pylori strains,isolated from the antral biopsies of 49 patients with dyspepsia,gastritis,gastric ulcer,duodenal ulcer, etc., were evaluated by elisa(upr)to determine the di...

Linear IgA bullous dermatosis (LABD) is characterized by linear binding of at the epidermal basement membrane zone. The major autoantigens for LABD are 120 kDa LAD-1 and 97 LABD97, which shedding products BP180. In general, autoantibodies to them detected immunoblotting using cell extract or culture medium keratinocyte lines, such as HaCaT cells, but they not highly sensitive. Therefore, we sou...

Abstract Purpose The glycolytic enzyme enolase plays important role in the pathogenesis of Candida albicans infection and has been also considered as a promising molecular marker for diagnosis invasive candidiasis. This study aimed to investigate location secretion features (CaEno) with couple specific monoclonal antibodies (mAbs). Methods Two mAbs named 9H8 10H8 against CaEno were generated by...

A murine monoclonal antibody generated against staphylococcal alpha-toxin was shown to react only with the monomeric (native), 3S form of the toxin. A sensitive sandwich enzyme-linked immunosorbent assay (ELISA) constructed with this antibody permitted detection of 0.25 to 0.5 ng of native toxin per ml. Toxin oligomers formed either by heat aggregation in solution, on target erythrocyte membran...

Cell-based analytics for the detection of the beta1-adrenoceptor autoantibody (beta1-AAB) are functional, yet difficult to handle, and should be replaced by easily applicable, routine lab methods. Endeavors to develop solid-phase-based assays such as ELISA to exploit epitope moieties for trapping autoantibodies are ongoing. These solid-phase-based assays, however, are often unreliable when used...