Introduction Shake flask cultivation is nowadays a routine technique during process development for mammalian cell lines. During shaken culture, changes in agitation velocity, shaking diameter or shake flask size affect the hydrodynamics in the shake flask. This might be reflected in the growth of the cultured cells. Process parameters such as power input, mixing time, fluid velocity etc. have ...

Aim To characterize human oral mucosa middle interstitial tissue fibroblasts (hOMFs) and their application in the cultivation of epithelial sheets. Methodology hOMFs were cultured with methylcellulose to form cell clusters. hOMFs amplified in adhesive culture were analyzed by flow cytometry, and were found to differentiate into multiple cell types suitable for the cultivation of human corneal...

Polysialic acid (polySia) is a promising molecule for various medical applications (e.g., treatment of inflammatory neurodegenerative diseases). In this study a complete production process for human-identical α-(2,8)-linked polySia was developed using a disposable bioreactor for cultivation of Escherichia coli K1 and single-use membrane adsorbers for downstream processing (DSP). The cultivation...

Background: Heterogeneity in recombinant protein quality (e.g. glycosylation) can result from a) changes in cultivation conditions during a fermentation process, b) product degradation due to differing residence times of product molecules in the supernatant, c) heterogeneities in intracellular PTM (post transcriptional modification) machinery, or from a combination thereof. To minimize heteroge...

Permeation of a drug through the cornea may be studied in vitro by using either the excised cornea of slaughtered animals or an organotypic cornea construct [1]. To compare drug permeability data reported in the literature for excised porcine cornea [2] with those from cornea constructs, the cultivation of an organotypic porcine cornea is required. The porcine cornea consists of three major cel...

A microfluidic device for microbial single-cell cultivation of bacteria was modeled and simulated using COMSOL Multiphysics. The liquid velocity field and the mass transfer within the supply channels and cultivation chambers were calculated to gain insight in the distribution of supplied nutrients and metabolic products secreted by the cultivated bacteria. The goal was to identify potential sub...

The primary culture bone marrow (PCBM) cells are often used in different cytological investigations. Here we study the behavior of porcine unstimulated PCBM cells during the cultivation. DNA cytophotometry analyses revealed that immature white blood cells (WBC) such as lymphoblasts, monoblasts and myeloid cells during cultivation process reduced the DNA content in the nuclei. This resulted in d...

Medicinal mushrooms have profound health-promoting benefits. Recently, a number of substances of mushroom origin have been isolated, identified and shown to have physiological activities, such as antitumor, immunomodulating, cardiovascular, antihypercholesterolemia, antibacterial, antiviral, antiparasitic, hepatoprotective, and antidiabetic activities. Currently, commercial products from medici...

Numerous studies have so far been conducted on the cultivation of Mycobacterium leprae and Mycobacterium lepraemurium. However, no media-bound method of cultivation has been established. A number of investigators have reported that M. lepraemurium may be cultivated by cell culture technics. The method employed by Chang (1. 2. 3 ), Chang and Neikirk (4), Chang, Anderson and Vaituzis (~), in whic...