We have developed a procedure to amplify mRNA into sense RNA (sRNA) so as to create a regenerating biorepository representing the complex mRNA profile in the original sample. The procedure exploits the template-switching activity of reverse transcriptase to incorporate RNA polymerase binding sites upstream of single-stranded cDNA (ss cDNA). Limited PCR was used for double-stranded DNA (dsDNA) s...

introduction: the estrogen receptors are potential candidates in prognosis and particularly in predicting hormone response therapy in breast cancer. the aim of this study was to measure erα gene expression due to study of the association between erα expression, age and menopause of iranian women with breast cancer. methods: 21 adenocarcinoma cases with grade 2 and 10 normal samples were screene...

vitiligo is a pigmentation disorder in which inflammatory mediators such as cytokines have a pivotal role in disease's pathogenesis. interleukin 17 (il-17a) is a proinflammatory cytokine which is involved in the induction of several proinflamatory mediators such as cyclooxygenase 2 (cox2). the aim of this study was to investigate the gene expression of il-17 and cox2 in peripheral blood leukocy...

objective(s):  being secreted by the pineal gland, melatonin induces cell proliferation in normal cells and induced apoptosis in cancer cells. the purpose of this study was to investigate effects of melatonin on main components and the expression of apoptotic genes in vitrified-thawed testicular germ cells of 6- day–old mice.  materials and methods: testes of neonate balb/c mice were vitrified-...

A quantitative real-time PCR (QPCR) method was developed to detect and quantify the amount of Solenopsis invicta virus (SINV) infecting individual ants of S. invicta. The two-step method utilized a gene-specific oligonucleotide primer targeting the SINV RNA-dependent RNA polymerase (RdRp) for cDNA synthesis. Dithiothreitol used in the cDNA synthesis step was found to significantly decrease the ...