The differential display reverse transcriptional polymerase chain reaction (DD-RT-PCR) was used to hunt for cDNA fragments specifically expressed by taxol treatment of HeLa cells. Forty-eight cDNA clones were differentially displayed through the experiments. The cDNA fragments obtained were separately spotted onto glass slides to prepare a tailor-made DNA chip. The gene expression pattern of di...

To move microarray technology into the diagnostic realm, the impact of technical parameters, such as sample preparation and RNA extraction, needs to be understood and minimized. We evaluated the impact of two RNA extraction methods, DNase treatment and the amount of hybridized cDNA probe, on the outcome of microarray results. The results for both RNA extraction methods were comparable, although...

cDNA microarray-based CGH (Microarray-CGH) is a useful technique for detecting genomic aberrations with a high resolution. However, the criteria for determining a genomic alteration have not been determined. We evaluated the genome-wide measurement of copy number of each gene in normal gastric and placenta tissues with both sex-matched, direct and sex-mismatched, indirect designs using 17K cDNA...

fadR is a transcription factor which has a Helix-turn-Helix motif [5], one of most common prokaryotic transcription factor. It regulates metabolic pathway such as the fatty acid biosynthesis and degradation pathways, glyoxylate pathway and possible role in regulation of amino acid biosynthesis directly or indirectly [1, 2, 3, 4]. By use of cDNA microarray one can search for regulation of transc...

activated macrophages have been classified into classical (m1) and alternative (m2) macrophages. we aimed to establish a method to yield enough number of macrophages to analyze their molecular, biological and immunological functions. we used drugs; adjuvant albumin from chicken egg whites—imject alum (ova- alum) and ova complete freund adjuvant (ova-cfa), to induce macrophages to m2 and m1 resp...

dna microarray is a powerful approach to study simultaneously, the expression of 1000 of genes in a single experiment. the average value of the fluorescent intensity could be calculated in a microarray experiment. the calculated intensity values are very close in amount to the levels of expression of a particular gene. however, determining the appropriate position of every spot in microarray im...

BACKGROUND DNA microarray technology has permitted the analysis of global gene expression profiles for several diseases, including cancer. However, standard hybridisation and detection protocols require micrograms of mRNA for microarray analysis, limiting broader application of this technology to small excisional biopsies, needle biopsies, and/or microdissected tissue samples. Therefore, linear...

Gene expression and its regulation are very important to understand the behavior of cells under different conditions. Various techniques are used nowadays to study gene expression, but most are limited in terms of providing an overall picture of the expression of the whole transcriptome. DNA microarrays offer a fast and economic research technology, which gives a full overview of global gene ex...

This review paper, is aimed at biological researchers who are interested in or have begun to use cDNA microarrays for their investigations. Large microarray studies typically involve a multidisciplinary team with various groups performing different aspects of the same experiment. This approach means that microarrays are less accessible to new researchers than more traditional biological techniq...