BACKGROUND CD11b/CD18 is a key adhesion receptor that mediates leukocyte adhesion, migration and immune functions. We recently identified novel compounds, leukadherins, that allosterically enhance CD11b/CD18-dependent cell adhesion and reduce inflammation in vivo, suggesting integrin activation to be a novel mechanism of action for the development of anti-inflammatory therapeutics. Since a numb...

Advances in fluorescence-activated cell sorter technology have brought about multicolor analysis of cell phenotypes. To clarify the phenotypes of human hematopoietic stem cells (HSCs), we initially prepared novel antibodies against CD34 and labeled one of them (4A1) with allophycocyanin (APC). With this, we analyzed the phenotypes of CD34+ HSCs and showed that primitive HSCs or CD34+CD33- cells...

Pretreatment of endothelial cells with cytokines enhances the adherence of leukocytes, a process that is mediated by surface proteins expressed on both cell types. A three-dimensional model system for the simultaneous determination of leukocyte adherence and migration was used to study the contribution of CD11/CD18, endothelial leukocyte-adhesion molecule-1 (ELAM-1) and VLA-4 in neutrophil and ...

All-trans retinoic acid (t-RA) administration leads to complete remission in acute promyelocytic leukemia (APL) patients by inducing growth arrest and differentiation of the leukemic clone. In the present study, we show that t-RA treatment dramatically induced type II transglutaminase (type II TGase) expression in cells carrying the t(15;17) translocation and expressing the PML-RARalpha product...

Patients with chronic kidney disease (CKD) and patients on dialysis are at risk for serious infectious complications. This is partly due to leukocyte dysfunction, which is a consequence of both uremic retention solutes and of dialysis. Paper I describes functions of in vivo extravasated monocytes and neutrophils (CD11b/CD18 expression, respiratory burst and apoptosis) from patients on highflux ...

The C D l l b/CD18 heterodimeric surface antigen is expressed exclusively on human monocytes, macrophages, granulocytes, and natural killer cells. During differentiation of myeloid cell lines, CDl lb steady state messenger RNA levels increase significantly; we show here that CDl lb transcription rates increase commensurately. A 1.7-kb fragment of C D l l b 5’ flanking sequence directs expressio...

The integrin lymphocyte function-associated antigen 1 (LFA-1; CD11A/CD18; ITGAL/ITGB2), is a key regulator of lymphocyte trafficking, activation and prolonged lymphocyte residence in lymph nodes (LNs) (Reichardt et al, 2013); yet, little is known about the regulation of ITGAL (CD11A) and ITGB2 (CD18) transcription. Chronic lymphocytic leukaemia (CLL) cells strongly depend on the lymphoid microe...

OBJECTIVE The intercellular adhesion molecule-1 (ICAM-1/CD54) and its ligand, CD11a/CD18, mediate endothelial adhesion of leukocytes and their consecutive transmigration. Anti-inflammatory effects of statins are considered to be exerted in part through inhibition of leukocyte-endothelial interactions. We investigated the in vivo effects of simvastatin treatment in hypercholesterolemic patients ...