نتایج جستجو برای: cccdna

تعداد نتایج: 249  

2015
Madina Karimova Niklas Beschorner Werner Dammermann Jan Chemnitz Daniela Indenbirken Jan-Hendrik Bockmann Adam Grundhoff Stefan Lüth Frank Buchholz Julian Schulze zur Wiesch Joachim Hauber

Current antiviral therapies cannot cure hepatitis B virus (HBV) infection; successful HBV eradication would require inactivation of the viral genome, which primarily persists in host cells as episomal covalently closed circular DNA (cccDNA) and, to a lesser extent, as chromosomally integrated sequences. However, novel designer enzymes, such as the CRISPR/Cas9 RNA-guided nuclease system, provide...

2014
Su-Ru Lin Hung-Chih Yang Yi-Ting Kuo Chun-Jen Liu Ta-Yu Yang Ku-Chun Sung You-Yu Lin Hurng-Yi Wang Chih-Chiang Wang Yueh-Chi Shen Fang-Yi Wu Jia-Horng Kao Ding-Shinn Chen Pei-Jer Chen

Persistence of hepatitis B virus (HBV) covalently closed circular DNA (cccDNA) under current antiviral therapy is a major barrier to eradication of chronic hepatitis B (CHB). Curing CHB will require novel strategies for specific disruption of cccDNA. The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system is a newly developed tool for site-specific cleavage of DNA tar...

Journal: :Journal of Clinical and Translational Hepatology 2019

2014
Hei-Man Vincent Tang Wei-Wei Gao Chi-Ping Chan Kin-Hang Kok Dong-Yan Jin

Background Chronic hepatitis B virus (HBV) infection occurs in over 400 million people worldwide, 15-40% of whom will terminally develop severe liver diseases including hepatocellular carcinoma. Although development of HCC is a multi-step process, high HBV DNA level is a major risk factor for disease progression. Transcription of HBV from the cccDNA template is essential for its replication and...

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