We report here the complete genome sequence of Candida tropicalis no. 121. C. tropicalis no. 121 is a high-RNA-producing strain obtained by mutagenesis in our laboratory. The complete genome sequence was determined using the Illumina HiSeq 2000 and contains 6,415 genes. The genome size of C. tropicalis no. 121 is >15.3 Mb.

Biosurfactants are amphipathic molecules with both hydrophilic and hydrophobic moieties and are surface active agent that are produced extracellularly or as a part of cell membrane by bacteria, yeast and fungi. In the present study, six different microorganisms were isolated from oil contaminated soils. Screening of biosurfactant producing potential strain was done using oil spreading method, o...

The performance of the Etest for testing the susceptibilities to caspofungin (MK-0991) of 726 isolates of Candida spp. was assessed against the National Committee for Clinical Laboratory Standards (NCCLS) microdilution broth method. The NCCLS method employed RPMI 1640 broth medium, and MICs were read after incubation for 48 h at 35 degrees C. MICs were determined by Etest for all 726 isolates w...

conclusions potent antifungal activity, make this plant an effective replacement treatment for fungal infections or fungal strains that are resistance to synthetic antifungals. results thirty-two components were identified in vitex agnus-castus eo. the main compound was alpha-pinene (19.48%). the total phenolic content of eo was determined as 82.26 ± 5.94 mg gallic acid equivalent (gae)/g eo. t...

Candida yeasts are the dominant fungi in the healthy human microbiome, but are well-known for causing disease following a variety of perturbations. Evaluation of fungal populations from the healthy human gut revealed a significant negative correlation between the foodborne yeast, Debaryomyces hansenii, and Candida species. D. hansenii is reported to produce killer toxins (mycocins) effective ag...

Candida tropicalis grows on soluble starch, corn, and cassava powders without requiring that these substrates be previously hydrolyzed. C. tropicalis possesses the enzyme needed to hydrolyze starch, namely, an alpha-amylase. That property has been used to develop a fermentation process whereby C. tropicalis can be grown directly on corn or cassava powders so that the resultant mixture of biomas...

Fluconazole disk-diffusion susceptibility was evaluated in 230 blood isolates and 344 non-blood clinical isolates of Candida spp. collected in 2002 at National Taiwan University Hospital. Up to 93.5% of blood isolates were susceptible to fluconazole, 3% were susceptible dose-dependent, and 3.5% were resistant. The minimum inhibitory concentrations at which 50% of tested isolates were inhibited ...

The paradoxical growth (PG) to echinocandins has been observed in vitro in Candida and Aspergillus species. Its mechanisms are incompletely understood, although activation of calcineurin and chitin biosynthesis pathways have been implicated. Importantly, the in vivo relevance of PG is not well defined as there is paucity of both animal model and clinical data on the implications of this phenome...

We have recently shown that 14a-demethylation-deficient cells of Candida albicans are subject to growth arrest by 0.24 M acetate in a yeast extract-peptone-glucose medium and that the minimum concentration of an azole antifungal agent required for total inhibition of sterol 14a-demethylation (MDIC for minimum demethylation-inhibitory concentration) is practically identical to its MIC determined...

Phenotypic switching allows for rapid transitions between alternative cell states and is important in pathogenic fungi for colonization and infection of different host niches. In Candida albicans, the white-opaque phenotypic switch plays a central role in regulating the program of sexual mating as well as interactions with the mammalian host. White-opaque switching is controlled by genes encode...