Abstract Introduction Calmodulin (CaM) is a highly conserved mediator of calcium (Ca2+) dependent signalling. Its flexible structure allows CaM to bind and modulate many targets, including cardiac ion channels. Genotyping has revealed several mutations associated with congenital disorders heart rhythm, known as long QT-syndrome (LQTS). LQTS patients suffer from prolonged ventricular recovery ti...

TOPIC: Lung Cancer TYPE: Original Investigations PURPOSE: Non-small cell lung cancer (NSCLC), one of the leading causes cancer-related death, has a low 5-year survival rate owing to inevitable acquired resistance toward antitumor drugs, platinum-based chemotherapy, and targeted therapy. Epidermal growth factor (EGF)-EGF receptor (EGFR) signaling activates downstream events phospholipase C/inosi...

We developed a method to evaluate the activity of Na+–Ca2+ exchanger (NCX) and sarco-endoplasmic reticulum Ca2+-ATPase (SERCA) with fluorescence microscopy in mouse ventricular cardiomyocytes. In non-beating cardiomyocytes, α-adrenoceptor stimulation by phenylephrine caused decrease cytoplasmic Ca2+ concentration, which was inhibited SEA0400, an NCX inhibitor, but not cyclopiazonic acid, SERCA ...

Type 1 ryanodine receptor (RyR1) releases Ca2+ ions from the sarcoplasmic reticulum of skeletal muscle to initiate contraction. Multiple endogenous and exogenous effectors activate RyR1. Cryo-electron microscopy has identified binding sites three co-activators Ca2+, ATP caffeine, however, mechanism co-regulation synergy between these activators remains be determined. Here, we tested hypothesis ...