Introduction A unilateral external fixator comprises of a collection of pins, clamps and sidebars to form a construct on one side of the limb that can be used to stabilise bone fractures and promote healing. An external fixator can allow the operator to alter the flexibility of the fixation. In the presence of flexible fixation, fracture healing happens through the creation of callus, which mec...

Creating transgenic plants is invaluable for the genetic analysis of sugar beet and will be increasingly important as sugar beet genomic technologies progress. A protocol for Agrobacterium-mediated transformation of sugar beet is described in this chapter. Our protocol is optimized for a sugar beet genotype that performs exceptionally well in tissue culture, including the steps of dedifferentia...

Barley is an important cereal crop worldwide. Its genetic transformation now limited to very few cultivars because of the high genotype dependence embryogenic callus. To reveal key genes or factors controlling callus induction and plantlet regeneration in barley, we compared transcriptomic profiles immature embryos Golden Promise ZU9, which differed dramatically efficiency transformation. The s...

BACKGROUND AND PURPOSE Parathyroid hormone (PTH) has attracted considerable interest as a bone anabolic agent. Recently, it has been suggested that PTH can also enhance bone repair after fracture and distraction osteogenesis. We analyzed bone density and strength of the newly regenerated mineralized tissue after intermittent treatment with PTH in rabbits, which undergo Haversian bone remodeling...

The paper studies the effect of RVI biocomposite material belonging to group osteoplastic materials, RV-2 immunomodulator – a synthetic dipeptide inducing an immunocorrective effect, and combinations these drugs on reparative histogenesis bone tissue in femoral fractures rats. It was found that remodeling primary callus into secondary one fracture studied animals diverse nature. This process mo...

Osteogenesis imperfecta is a rare inherited disorder of connective tissue which may present with recurrent fractures which are prone to nonunion and malunion resulting in deformity. Some patients develop osteoarthritis of the hip. Formation of hyperplastic callus after recurrent fractures may deform the shape of the femur and preclude the use of standard implants at joint replacement. Replaceme...

In five cultivated rice varieties e.g., Nonabokra, PNR 381, Taipei 309, Koshika and TKM 11 the response in the initiation of callus and its size varied greatly among varieties, salt concentrations (NaCl and Na2SO4) and their interaction. Better regeneration ability of Nonabokra, Koshika and TKM 11 was found to be correlated to small size callus with a high concentration of Na2SO4. The regenerat...

In order to investigate the genetic variation in tissue culture response and to ®nd the cultivars with high regeneration ability for genetic transformation, twenty®ve turf-type tall fescue (Festuca arundinacea Schreb.) cultivars, including many elite ones released recently, were evaluated for their callus induction and plant regeneration responses. Callus induction was initiated from mature see...

Bone fracture healing is a complicated physiological regenerative process initiated in response to injury and is similar to bone development. To demonstrate whether an exogenous supply of parathyroid hormone-related protein (PTHrP) helps in bone fracture healing, closed mid-diaphyseal femur fractures were created and stabilized with intramedullary pins in eight-week-old wild-type (WT) PTHrP+/+ ...

Ascorbate oxidase from pumpkin (Cucurbita sp.) was purified from a commercially available preparation. A single polypeptide band with M(r) 64,000 was detected after sodium dodecylsulfate-polyacrylamide gel electrophoresis of the purified enzyme. In double immunodiffusion tests, antiserum against the purified preparation formed a single precipitin line with the crude extract from pumpkin fruit t...