A rapid and efficient protocol was developed for inducing indirect organogenesis using leaf explants of Vitex negundo L. Explants were cultured on MS with different concentrations of 2,4-D and IAA in combination with BAP for callus induction. The frequency of callus induction increased with increasing concentration of IAA (0.3 mg/l) and BAP (0.3 mg/l) at optimal level. The shoot buds appeared e...

Three basal plant tissue culture media, namely, N6, MS, and modified Y3, were compared to optimize micropropagation protocol for E. guineensis. Full strength media were used separately to regenerate plantlets directly using immature zygotic embryos (IZEs), and through somatic embryogenesis of calli obtained from IZEs. The plantlets regenerated by direct regeneration on three media were examined...

An experiment was conducted to study the effect of growth regulators on callus induction in rice embryo culture. In this experiment, caullogenesis was initiated from the matured seeds of seven indica rice varieties viz., TRY 1, TRY 2, Pokkali, CSR 10, W.Ponni, BPT 5204 and IR 29. The medium used for this callus induction was Murashige and Skoog (1962) commonly referred as MS medium with six dif...

Background and objectives: A modern biotechnological technique to obtain useful natural products from plants is to isolate them from their callus. Zataria multiflora is a bushy herb of the Lamiaceae family known for its stimulant, antibacterial, antioxidant and expectorant effects in Iranian folk medicine.  The present study has investigated the induction of callus tis...

The effects of plant growth regulators were examined in order to optimize the callus induction, regeneration, and proliferation of lisianthus (Eustoma grandiflorum). In vitro leaves provided the explants for callus induction. Explants were cultured on Murashige and Skoog (MS) medium with different concentrations of indole-3-acetic acid (IAA), 1-naphthaleneacetic acid (NAA), and 2,4-dichlorophen...

Callus growth and development, a crucial process in plant propagation, is involved hormonal balance abundant gene regulation. MiRNAs are key regulators the of cell differentiation development. MiR397 was identified as participating growth, response to stress, it regulated by targeting LAC gene. The regulatory function miR397 during callus development not clear Liriodendron. In this study, LhmiR...

PIWOWARCZYK BARBARA, PINDEL ANNA, MUSZYŃSKA EWA. 2016. Callus Induction and Rhizogenesis in Lathyrus sativus L. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 64(1): 123–128. Grass pea (Lathyrus sativus L.) is a leguminous plant distinguished by great resistance to abiotic and biotic stresses that can become a valuable source of protein feed in the nearest future. Howev...

leaf and root explants from 10 to 20 day– old seedlings of plantago lanceolata, were cultured on ms, msh, nt and b5 basal media with various concentrations of different plant growth regulators. the best callus induction was obtained with 0.4 mgl-1 2,4-d in msh i with 400 callus index. callus growth was significantly stimulated (p=0.05)with 0.8 mgl-1 2,4-d and 0.1 mgl-1 kin in msh ii, with 7...