the present study was aimed at the development of a somoclonal variant and isozyme marker for phyllanthus amarus schum & thonn using inter-nodal segments and the enzyme peroxidase. maximum callus proliferation was obtained on murashige and skoog’s medium supplemented (ms) with 1.0 mg/l of 2,4-dichlorophenoxyacetic acid. three weeks-old pale yellowish white semi-friable callus was used for o...

INTRODUCTION Normal fracture healing is a complex, multi-step process involving cellular events influenced and regulated by local and systemic factors. The most common biological failure in fracture healing involves an improperly formed callus during the first weeks following fracture. Recent advances in understanding the regulatory factors controlling fracture healing have suggested that a num...

3D Biomimetic Calcified Cartilaginous Callus Based on the natural bone repair process, a biomimetic calcified cartilaginous callus (PHS) is prepared using calcium ion chelation and printing technology to promote regeneration of large-segment defects. PHS can both type H vessels formation osteoclast by facilitating expression osteogenesis-promoting coupling factors. More details be found in arti...

Root-knot nematodes (RKNs; Meloidogyne spp.) induce feeding cells (giant cells; GCs) inside a pseudo-organ (gall) from still unknown root cells. Understanding GCs ontogeny is essential to the basic knowledge of RKN-plant interaction and to discover novel and effective control strategies. Hence, we report for the first time in a model plant, Arabidopsis, molecular, and cellular features concerni...

Callus formation is a critical step for successful fracture healing. Little is known about the molecular composition and mineral structure of the newly formed tissue in the callus. The aim was to evaluate the feasibility of small angle x-ray scattering (SAXS) to assess mineral structure of callus and cortical bone and if it could provide complementary information with the compositional analyses...

The aim of this study is to clarify the effect of low intensity pulsed ultrasound (LIPUS) on shortening of the fracture healing period and endochondral ossification during the fracture healing process. We first established a model of aging-related delayed union fractures consisting of aged mouse (C57BL/6J; 40 weeks old) with closed femur fractures. We compared the healing process of 40-week-old...

The seeds of ten different genotypes of chickpea (Cicer arietinum L.) originated from Egypt, ICARDA and Saudi Arabia, were used to screen their tissue culture response on various media combination. MS media supplemented with 1, 2 and 4 mg of each of IBA and transzeatin /l, were tested. The results showed that stem results were far better from leaves explants. The callus induction ranged from 6....

The experiment was conducted during May to December 2008 in the Biotechnology Laboratory of Bangladesh Agricultural University, Mymensingh to observe the callus induction, regeneration potentiality and to establish a suitable in vitro plantlet regeneration protocol of Corchorus olitorius. MS medium supplemented with different phytohormone concentrations and combinations were used to observe the...

Explants of leaves, petals, anthers, styles and ovaries of C. speciosus, C. cancellatus, C. gilanicus and C. almehensis were cultured on LS media with three different combinations of NAA and BAP in the dark. Callus and neoformant organs (leaf and corm-like structure and ordinary and contractileroots) were fomd on some of these explants. Corm explants from these species were also cultured on...