Abstract Leucojum aestivum is a valuable and endangered plant species with bulb scales best suited as explants in micropropagation. In the current study, its micropropagation was investigated by using two different various concentrations combinations of growth regulators (PGRs). Bulbs were first disinfected benomyl® for 5 hours. After meeting chilling requirements, two-scale four-scale provided...

Baccharis dracunculifolia DC. is a pioneer plant native from Brazil that has great importance due to its secondary metabolites (essential oil and Brazilian green propolis) potential in the recovery of degraded areas. The main goal this study was evaluate propagation techniques B. by sexual reproduction asexual male female stem cuttings. For reproduction, individuals natural population were peri...

In the present study, the effect of 2,4-dichlorophenoxyacetic acid (2,4-D) on callus cultures of Artemisia annua were evaluated in terms of its growth, chlorophyll, carotenoid, protein, phenol and flavonoid content and possible genetic variations using ISSR and RAPD markers. Callus cultures grown on MS medium supplemented with three different 2,4-D concentration (1.0, 2.0 and 3.0 mg 2,4-D L MS ...

BACKGROUND The somatic embryogenesis tissue culture process has been utilized to propagate high yielding oil palm. Due to the low callogenesis and embryogenesis rates, molecular studies were initiated to identify genes regulating the process, and their expression levels are usually quantified using reverse transcription quantitative real-time PCR (RT-qPCR). With the recent release of oil palm g...

Background: In Algeria, date palm is currently confronted to the Bayoud disease. Biotechnological tools such as protoplasts fusion can appear as an alternative to ensure rapid multiplication and improvement of this species. Objectives: Callogenesis induction in protoplasts isolated from embryogenic callus of three date palm cultivars. Materials and Methods: Some factors influencing the isolatio...

The present study was carried out to evaluate the effects of light and plant growth regulators on callus induction and explant browning in date palm cultivar, Estameran in in vitro condition. The explants were pretreated by an antioxidant combination (100 mgl-1 citric acid and 150 mgl-1 ascorbic acid) for 3 days in 5 °C. Then, the  leaf explants  were cultured in Murashige and Skoog medium (MS)...

background: human granulocyte colony-stimulating factor (hg-csf) can serve as valuable biopharmaceutical for research and treatment of the human blood cancer. transplastomic plants have been emerged as a new and high potential candidate for production of recombinant biopharmaceutical proteins in comparison with transgenic plants due to extremely high level expression, biosafety and many other a...