It is well-documented that organic compounds form strong complexes with most metals in aquatic systems, and that seawater is a complex medium which contains a large variety of organic and inorganic ligands, including colloidal matter. We suggest that most trace metals are complexed in seawater and that some inorganic metals complexes are either labile or not stable. In contrast, metal-organic c...

An official liquid chromatographic method for the analysis of erythromycin and related substances, which is based on a polymer reversed-phase, is described in the European Pharmacopoeia and in the United States Pharmacopeia. The pH of the mobile phase used in this system is 9.0. Recent advanced technology has led to the introduction of a new generation of silica-based reversed-phase column pack...

A simple, selective, accurate high Performance Liquid Chromatographic (HPLC) method was developed and validated for the analysis of Paracetamol and Ibuprofen. Chromatographic separation achieved isocratically on a C18 column [Use Inertsil C18, 5μ , 150 mm x 4.6 mm] utilizing a mobile phase of acetonitrile/phosphate buffer (60:40, v/v, pH 7.0) at a flow rate of 0.8 ml/min with UV detection at 26...

This research paper describes simple, specific, accurate and precise reverse phase high pressure liquid chromatographic method for the simultaneous determination of Montelukast sodium and Bambuterol hydrochloride in combined dosage form. The sample was analyzed by reverse phase C18 column (Phenomenex C18, 5μ, 250mm x 4.6mm) as stationary phase; methanol: acetonitrile : 1% trichloroacetic acid i...

A simple, sensitive isocratic reversed-phase HPLC method for detecting and quantifying ciprofloxacin in pharmaceutical dosage forms has been developed validated according to ICH guidelines. In the method, separation was achieved on a C18 column with mobile phase acetonitrile-water at ratio of (80:20). The pH adjusted 2.7 using 85% phosphoric acid ultraviolet detection 275.0 nm. flow rate 1.7 mL...

Purpose: To develop and validate a sensitive and specific HPLC assay for cladribine (CdA) in plasma for pharmacokinetic studies in rats. Methods: CdA and the internal standard AZT were purchased from Sigma-Aldrich Chem. The HPLC system consisted of a Shimadzu LC-9A pump, a 3 μm, 250 x 2.0 mm I.D. high speed C18 column (Jupiter), preceded by a 5 μm 4 x 4 mm I.D. C18 guard column (Licrocart), an ...

     A special type of silica-based columns has been recently introduced into the market which is called narrow-bore columns. They have lower internal volume than the standard high-performance liquid chromatography (HPLC) columns and thus reduce the solvent consumption by almost 80%.    A simple, accurate and environmentally friendly reversed phase- HPLC (RP-HPLC method) which could be used in ...

A rapid liquid chromatographic method was developed for the determination of oxatomide in its finished active pharmaceutical ingredient form and in the presence of its process impurities. The method was developed on a sub 2 µm Hypersil Zorbax XDB C18 column (30 × 4.6 mm, i.d., 1.8 µm). The rapid method employed a gradient mobile phase consisting of solvent A: 0.01 M tetrabutylammonium hydrogen ...

The aim of this study was to develop an HPLC method for simultaneous determination of these active compounds and to apply this method to determine the dissolution of AML and OLM from a commercially available tablet. Valsartan (VAL) was used as an internal standard (IS). Separation of AML, OLM and VAL was performed using Phenomenex C18 column (Luna 5 μ, 100A, 250x4.6 mm; California, USA) protect...