The conversion of varphiX174 single-stranded DNA to duplex DNA by extracts of E. coli requires products of the E. coli DNA replication genes. By use of this complementation system, the dna C gene product has been purified from wild-type E. coli as well as from a dna C temperature-sensitive mutant. The latter preparations are temperature sensitive when compared to the wild-type gene product. The...

objective: traditional methods for storing microbial dna samples use the low-temperatures of 4 °c, -20°c or -80 °c. the dbc card is a new method of storing microbial dna at room temperature. this study evaluates the stability and determine the best storing method of bacterial dna on the dbc card. methods: in this study, we used a pair of primers from the conserved domain of 16srrna designed to...

Fresh-cut leafy greens contaminated with Escherichia coli O157:H7 have caused foodborne outbreaks. Packaging conditions, coupled with abusive storage temperatures of contaminated lettuce, were evaluated for their effect on the potential virulence of E. coli O157:H7. Shredded lettuce was inoculated with 5.58 and 3.98 log CFU E. coli O157:H7 per g and stored at 4 and 15°C, respectively, for up to...

We analyzed 100 Campylobacter spp. isolates (C. jejuni and C. coli) from Grenada, Puerto Rico and Alabama, which were collected from live broilers or retail broiler meat. We analyzed these isolates with four molecular typing methods: restriction fragment length polymorphism of the flaA gene (flaA-RFLP), multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and automated re...

Thermotolerant Campylobacter spp. are a major cause of foodborne gastrointestinal infections worldwide. The linkage of human campylobacteriosis and poultry has been widely described. In this study we aimed to investigate the prevalence, antimicrobial resistance and genetic diversity of C. coli and C. jejuni in broilers from Ecuador. Caecal content from 379 randomly selected broiler batches orig...

The presence of virulence factors (VFs) and mechanisms of quinolones and macrolide resistance was analyzed in Campylobacter spp. from a pediatric cohort study in Lima. In 149 isolates (39 Campylobacter jejuni and 24 Campylobacter coli from diarrheic cases; 57 C. jejuni and 29 C. coli from controls), the presence of the cdtABC and cadF genes and iam marker was established. Nalidixic acid, ciprof...

The amino acid-producing organism Corynebacterium glutamicum cannot utilize glycerol, a stoichiometric by-product of biodiesel production. By heterologous expression of Escherichia coli glycerol utilization genes, C. glutamicum was engineered to grow on glycerol. While expression of the E. coli genes for glycerol kinase (glpK) and glycerol 3-phosphate dehydrogenase (glpD) was sufficient for gro...

This study was conducted to determine the prevalence of Campylobacter spp. on fresh chicken carcasses at retail markets in four selected urban districts Hanoi, Vietnam. A total 107 carcass samples were collected. found 44 and accounted for a 41.1%. Among species detected, C. coli predominant spp., being detected 24 samples, followed by jejuni, 19 samples. One positive both jejuni coli. The high...

The survival of Campylobacter jejuni and Campylobacter coli at pH 7.0, 6.0, 5.0 and 4.0 for up to 24 h, and the induction of an acid adaptation response in 12 C. jejuni and 10 C. coli strains in early exponential or late stationary phases in tryptic soy broth (TSB) and Brucella broth were investigated. C. coli strains were more sensitive than C. jejuni in media adjusted to pH 5.0 or 4.0 with hy...