The effect of polyadenylic acid: polyuridylic acid complex (poly A: U) on immunization was investigated in mice vaccinated by killed Brucella melitensis cells suspended in incomplete adjuvant or in saline. Addition of 300 ,ug of poly A:U to vaccines rendered 3 X 108 B. melitensis cells in saline as immunogenic as 3 X 10"1 cells in oil adjuvant against a severe B. abortus challenge. In the mouse...

Brucellosis remains an endemic zoonotic disease in many developing countries, causing great harm to public health and devastating losses livestock. One of the main reasons for low effectiveness anti-brucellosis measures is lack reliable methods diagnosing infected animals throughout their lifespan. Classical serological tests, such as tube agglutination test, rose Bengal plate complement fixati...

Some anticytoplasmic protein monoclonal antibodies (MAbs) from mice immunized by infection with Brucella ovis cells have been obtained. One of these MAbs, BI24, was used to purify by immunoaffinity a protein with a pI of 5.6 and a molecular mass of 18 kDa. This protein was present in all of the rough and smooth Brucella species studied, but it could not be detected in Yersinia enterocolitica 09...

Granulocytic Ehrlichia infection in sheep is common in Norway in areas with Ixodes ricinus. In this study, 2 sheep flocks that had been grazing on I. ricinus infested pastures the previous season, were blood sampled after being housed indoors for nearly 6 months during wintertime. Thirty animals from each flock were examined for granulocytic Ehrlichia infection in the peripheral blood by blood ...

The effect of polyadenylic acid:polyuridylic acid complex (poly A:U) on immunization was investigated in mice vaccinated by killed Brucella melitensis cells suspended in incomplete adjuvant or in saline. Addition of 300 mug of poly A:U to vaccines rendered 3 x 10(8)B. melitensis cells in saline as immunogenic as 3 x 10(11) cells in oil adjuvant against a severe B. abortus challenge. In the mous...

The case is reported of a 7 year old Bedouin girl who presented with a three week history of fever and limp. Initial tests for rheumatoid factor, antinuclear antibodies, and brucella were negative. Two weeks after admission Brucella melitensis was cultured from bone marrow and synovial fluid samples. The patient improved after treatment with doxycillin and streptomycin, but radiographs of the r...

Although a "One Health" approach has been successfully implemented for emerging infectious zoonotic diseases with pandemic potential, we still lack a conceptual framework to address enzootic diseases like brucellosis. The vast majority of published brucellosis studies in the developing world rely solely on serology. An important shortcoming of brucellosis serology is the impossibility to infer ...

Brucellosis caused by various species of the genus Brucella is one most important zoonotic diseases global importance with veterinary, public health, and economic concerns. The study aimed to standardize IgM IgG-based iELISA detect anti-Brucella antibodies for serodiagnosis acute chronic human brucellosis. test was standardized using 1:320 dilution smooth lipopolysaccharide (sLPS) antigen from ...

Thrombotic thrombocytopenic purpura (TTP) is a severe disease, potentially fatal, if not diagnosed and treated promptly. TTP is clinically characterized by the pentad of thrombocytopenia, Coombs-negative hemolytic anemia, fever, renal abnormalities and neurological disturbances. Advances in recent years have delineated the molecular mechanisms of acquired and hereditary TTP.Many infectious orga...

BACKGROUND Several zoonotic diseases are known to constitute great impediment to livestock management and production worldwide, especially in developing countries where control measures are largely non-existent. This study sets out to investigate the occurrence of toxoplasmosis, neosporosis and brucellosis among cattle herds in Oyo State, southwest Nigeria. MATERIALS AND METHODS A cross-secti...