We have developed an assay system for measuring lactate in whole blood, consisting of a single-use strip of an enzyme-coated electrode and a small meter. The electrode strip is made of three plastic films: a cover sheet, a spacer, and an insulation layer printed with electrodes that are coated with lactate oxidase (EC 1.1.3.x) and ferricyanide as an electron mediator. The meter measures the mag...

REASONS FOR PERFORMING STUDY The transverse facial venous sinus (TFVS) can be used for blood collection in horses, but information on the validity of blood values from this site is limited. OBJECTIVES To determine if packed cell volume (PCV), total solids (TS) and blood lactate concentrations in blood drawn simultaneously from a TFVS and jugular vein of critically ill horses are correlated an...

Forty-four critically ill patients with or without adult respiratory distress syndrome (ARDS) were studied in an attempt to define critical levels of oxygen delivery. Blood lactate was used as the indicator of tissue hypoxia independent of cardiac output. Survival was good (55 percent) and blood lactate near normal for those with oxygen delivery more than 8 ml/kg/min. Below this level, survival...

1. Circulating concentrations of intermediary metabolites have been measured after administration of 50 mg of phenformin to normal subjects. 2. Phenformin caused a significant increase in blood lactate, alanine and the lactate/pyruvate ratio but did not affect blood glucose or serum insulin concentrations. 3. There was a significant correlation between the increase in blood lactate concentratio...

Upon exposure to low Po(2), the red blood cells of most species, including humans, release increased amounts of ATP that ultimately serves as a regulator of vascular tone matching oxygen supply with demand. In pathological conditions such as malaria and sepsis, a maldistribution of perfusion exists with its severity often correlated with the extent of elevation of serum lactate frequently in th...

BACKGROUND Using finger-stick capillary blood to assess lactate from the microcirculation may have utility in treating critically ill patients. Our goals were to determine how finger-stick capillary lactate correlates with arterial lactate levels in patients from the surgical intensive care unit, and to compare how capillary and arterial lactate trend over time in patients undergoing resuscitat...

We have developed a dry reagent strip system for measuring lactate in whole blood. The test strip contains lactate oxidase (no EC number assigned), horseradish peroxidase (EC 1.11.1.7), and N-ethyl-N-(2-hydroxy-3-sulfopropyl)-m-toluidine. The system is designed to measure with a reflectometer the color that developed in the test strip, although the lactate concentration can be estimated without...

To assess early-onset differences in glucose metabolism in patients with sepsis during the first day of ICU hospitalization and evaluate these differences vis-à-vis shock resolution, we studied 10 (8 men; mean age+SD: 58+20 years) mechanically ventilated patients with a diagnosis of septic shock. On day 1 blood and microdialysis (MD) samples were collected twelve times per day (every 2 hours) f...

In this study, the authors attempted to determine whether the dynamics of blood lactate and glucose in wrestling depend on the weight class. Blood lactate and glucose curves during and after a wrestling match were determined. We also explained the dynamics of blood lactate and glucose in the context of recent glucose and lactate metabolism research. A sample of 60 youth wrestlers (15-20 years) ...