OBJECTIVES Twenty-five MRSA ST398 isolates from cases of bovine clinical mastitis and two isolates from farm personnel collected from 17 dairy farms in Germany were investigated for genetic relatedness, antimicrobial resistance and virulence properties. METHODS Genomic relationships were determined by ApaI PFGE, spa typing, SCCmec typing and dru typing. Antimicrobial resistance phenotypes wer...

In response to β-lactam chemotherapy, Staphylococcus aureus has acquired two resistance determinants: blaZ, coding for β-lactamase, which confers resistance to penicillins only, and mecA, coding for an extra cell wall cross-linking enzyme with reduced affinity for virtually all other β-lactams. The transcriptional control of both resistance determinants is regulated by homologous repressors (Bl...

This study aimed to investigate the prevalence, molecular characteristics and antibiotic resistance of Staphylococcus aureus isolates from yak butter in Tibet, China. A total 218 samples were collected retail stores Tibet screened for Staph. aureus. Furthermore, virulence genes, antimicrobial susceptibility, typing [pulsed-field gel electrophoresis (PFGE), multilocus sequence typing, staphyloco...

The presence of Staphylococcus spp. has increasingly been reported in food products and poses a public health threat. aim this study was to determine the diversity antibiotic resistance profiles isolates obtained from freshly harvested packed ready-to-eat mushrooms (n=432) handlers’ hands (n=150). A total 56 [46.4% (n=26) 53.6% (n=30) mushrooms] were recovered belonging 10 species. succinus (n=...

BACKGROUND In years past, methicillin-resistant S. aureus (MRSA) has been frequently detected in pigs in Europe, North America and Asia. Recent, yet sporadic studies have revealed a low occurrence of MRSA in Switzerland. In 2009, a monitoring survey of the prevalence and genetic diversity of methicillin-resistant S. aureus (MRSA) in slaughter pigs in Switzerland was conducted using methods reco...

S AND PAPERS OF LECTURES 11 BIOTECHNOLOGY 11 Špela Kos: GENE ELECTROTRANSFER FOR DNA VACCINATION 12 Helena Volk: PRODUCTION OF RECOMBINANT EFFECTOR PROTEINS FROM Verticillium nonalfalfa 13 Mojca Juteršek: TOWARDS MOLECULAR GENETIC DELINIATION OF Synechocystis SPECIES 14 Ester Stajič: DEVELOPMENT OF CABBAGE HAPLOID INDUCER LINE WITH GENOME EDITING 20 Katja Guček: CRISPR/Cas9 GENOME EDITING IN RA...

Sir, In a previous study, we identified a methicillin-resistant Staphylococcus epidermidis (MRSE) clone being present in nine different samples obtained from two cats hospitalized in a small animal clinic, an employee and the clinic environment. Members of this MRSE clone exhibited SCCmec type IV, dru type dt10a, multilocus sequence type ST5 and very similar or indistinguishable SmaI macrorestr...

Sir, The genes vga(A) and vga(C) and the most recently identified gene, vga(E), as well as the gene cfr, mediate transferable resistance to pleuromutilins in staphylococci. The vga genes encode ABC transporters, which also export streptogramin A antibiotics and lincosamides, and the cfr gene encodes a methyltransferase that confers additional resistance to phenicols, lincosamides, oxazolidinone...