Puerperal infection is a common complication during postnatal period in developing countries. Bacterial species, drug resistance, and genetic characteristics were investigated for a total of 470 isolates from puerperal infections in Bangladesh for a 2-year period (2010-2012). The most common species was Escherichia coli (n=98), followed by Enterococcus faecalis (n=54), Staphylococcus haemolytic...

The Dilúvio stream is part of an important watershed in the city of Porto Alegre, southern Brazil. It receives rain runoffs and domestic and hospital sewage, which carries a highly diverse microbial population. Some of these microorganisms may show resistance to different antimicrobials, acting as disseminators of resistance genes. This study characterizes the Gram-negative population present i...

AGGAATCCCATG-30 and CTX-M-R2, 50-GCCGTCTAAGGCGATAAAC-30, were used to amplify the entire allele (876 bp) for sequencing. This revealed that S. enterica serovar Senftenberg strain 9-4 produced CTX-M-3, which was first identified in Poland. Southern hybridization using the whole blaCTX-M gene as a probe showed that the gene was located on a plasmid of < 38 kb in size. Attempts to transfer this pl...

Studies of the transmission epidemiology of antimicrobial-resistant Escherichia coli, such as strains harboring extended-spectrum beta-lactamase (ESBL) genes, frequently use selective culture of rectal surveillance swabs to identify isolates for molecular epidemiological investigation. Typically, only single colonies are evaluated, which risks underestimating species diversity and transmission ...

Shigellosis is a major source of gastroenteritis throughout the world (14). Extended-spectrum -lactamases (ESBLs), including cefotaximases (CTX-M), confer resistance to extendedspectrum cephalosporins and significantly compromise the treatment options for shigellosis. Numerous ESBLs have been described among Enterobacteriaceae (2, 8, 13); however, only a single CTX-M-producing Shigella isolate ...

Stenotrophomonas maltophilia is a widespread environmental microorganism (1) that has emerged as a significant opportunistic pathogen (2) due to its intrinsic resistance to almost all available antibiotics. Apart from native -lactamases L1 and L2 (1), acquired extended-spectrum -lactamases (ESBLs) were also identified (3, 4), pointing to S. maltophilia as a potential reservoir. Furthermore, res...

Background: Atypical enteropathogenic Escherichia coli (aEPEC) is regarded as a globally emerging enteropathogen. aEPECs exhibit various level of resistance to a range of antibiotics, which is increasing alarmingly. The present study investigated the antimicrobial resistance of aEPEC isolates recovered from diarrheal patients, healthy carriers, animals, and raw meats. Results: Among 267 aEPEC i...

Kouta Hamamoto, Shuhei Ueda, Takehiko Toyosato, Yoshimasa Yamamoto, Itaru Hirai Laboratory of Microbiology, School of Health Sciences, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan; Japan Agency for Medical Research and Development/Japan International Cooperation Agency, Science and Technology Research Partnership for Sustainable Development, Tokyo, Japan; Nursing for Home Care...

1. Eckert C, Gautier V, Arlet G. DNA sequence analysis of the genetic environment of blaCTX-M genes. J Antimicrob Chemother 2006; 57: 14–23. 2. Cantón R, Coque TM. The CTX-M b-lactamase pandemic. Curr Opin Microbiol 2006; 9: 466–75. 3. Partridge SR, Hall RM. In34, a complex In5 family class 1 integron containing orf513 and dfrA10. Antimicrob Agents Chemother 2003; 47: 342–9. 4. Valverde A, Cant...

Day-old chicks from 3 hatcheries were placed on bedding paper and brought to a commercial broiler farm between January and July 2016. Sixty-six samples of the paper, which were stained with meconium droppings of the chicks, were collected and examined for isolation of cephalosporin-resistant Enterobacteriaceae. Cefotaxime (CTX)-resistant Klebsiella pneumoniae (1 isolate) and Enterobacter cloaca...