objective: classic cell culture and karyotyping is routinely used for prenatal detection of different chromosomal abnormalities. molecular cytogenetic techniques have also recently been developed and used for this purpose. quantitative florescence pcr using short tandem repeat (str) markers has more potential for high throughput diagnosis. marker heterozygosity in short tandem repeats (str) is ...

Random amplified microsatellite polymorphism (RAMP) is a PCR-based marker which uses a combination of two classes of markers: Simple sequence repeat (SSR) and Random amplified DNA polymorphism (RAPD) markers. RAMP has been demonstrated to be a potentially valuable molecular marker for the study of genetic relationships in cultivated plant species. The objective of this study was to optimize the...

Liver cancer is called the "king of cancer" because its extremely high mortality rate and lack effective treatment methods. Effective early diagnosis liver advance discovery period can effectively improve survival life cycle. Early be done by traditional US, CT, AFP level measurement other methods, or biological markers such as GPC-3, micro-RNA, detection some specific cell signal factors. Prec...

We report the identification of a molecular signature using the Scano-miR profiling platform based on the differential expression of circulating microRNAs (miRNA, miR) in serum samples specific to patients with very high-risk (VHR) prostate cancer (PCa). Five miRNA PCa biomarkers (miR-200c, miR-605, miR-135a*, miR-433, and miR-106a) were identified as useful for differentiating indolent and agg...

Past discussions of the relative strengths of nested case-control and case-cohort designs have not fully considered cohorts with stored biological samples in which biomarker analyses are planned. Issues related to biomarker analyses can affect an investigator's choice of design and the conduct of these two designs. The key issues identified are effects of analytic batch, long-term storage, and ...

X. Moles Lopez, O. Debeir, I. Salmon and C. Decaestecker 1 DIAPATH, Center for Microscopy and Molecular Imaging, Université Libre de Bruxelles, Rue Adrienne Bolland 8, B6041 Gosselies, Belgium 2 Laboratories of Image, Signal processing and Acoustics, Brussels School of Engineering, Université Libre de Bruxelles, 50, av. Franklin Roosevelt, B-1050 Brussels, Belgium 3 Department of Pathology, Era...

For centuries olive tree is an important crop in many Mediterranean countries because it provides appreciated oil with healthy properties. Th e lack of genomic tools, such as molecular markers or sequence information, has hindered the development of new cultivars adapted to the challenges that this species faces due to the change in modern cultivation practices, such as the increase in the numb...

Salivary biomarkers have important potential to facilitate breakthroughs in epidemiologic studies, management of emergency situations, and detection and surveillance of diseases by medical staff. During the last decade, an increasing number of studies on salivary biomarkers have been published as a consequence of the impressive development of new high-throughput technologies. Here, we present a...

many agriculturally important traits are complex, affected by many genes and the environment. quantitative trait loci (qtl) mapping is a key tool for studying the genetic structure of complex traits in plants. in the present study qtls associated with yield and agronomical traits such as leaf number, leaf length, leaf width, plant height, stem and head diameter were identified by using 70 recom...

This is a state-of-the-art review of the molecular genetics of pancreatic neoplasms. Although understanding of the molecular features underlying pancreatic neoplasms is still in its infancy, a strong emphasis on the relevance of these findings for the practicing surgical pathologist is provided. The application of molecular techniques has yielded a wealth of information that may soon enhance di...