Nanoparticles (NPs) have a number of unique properties associated with their ultrasmall size and exhibit many advantages compared with existing plant biotechnology platforms for delivery of proteins, RNA and DNA of various sizes into the plant cells (Arruda et al., 2015; Silva et al., 2010; Martin-Ortigosa et al., 2014; Mitter et al., 2017) [1], [2], [3], [4]. The data presented in this article...

The production of transgenic cells is a routinely process that allows to insert genes from plants, fungi, viruses, bacteria and even animals into cells. Genetic transformation requires penetration of the transgene through the cell wall. This process is facilitated by biological, chemical or physical methods. We present a short review of the state of the art of physical methods used for genetic ...

The efficiency of wheat biolistic transformation systems strongly depends on the bombardment parameters, the condition of the donor plant, and the plant genotype chosen for the transformation process. This paper analyzes the transformation efficiency of the 129 wheat sister lines generically called 'Bobwhite', originally obtained from the cross 'Aurora'//'Kalyan'/'Bluebird 3'/'Woodpecker'. A nu...

The class 2/type II clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 system has been used successfully for simultaneous modification of multiple loci in plants. Two general strategies have been applied to coexpressmultiple single guide RNAs (sgRNAs) to achieve multiplex gene editing in plant cells. One is to construct the multiple guide RNA expression cassettes into separa...

Objective: The most appropriate conditions for genetic transformation through direct (bioballistic) and indirect (Agrobacterium tumefaciens) systems in Paulownia elongata were established.
 Design/methodology/approach: Starting from vitro propagation both organogenesis, internodal stem segments with 0.5 to 1 cm length determined as the best explant. optimum dose selection media was be 15 m...