Thymidylate synthase (TS; EC 2.1.1.45) is an important cellular enzyme that converts dUMP to dTMP, which is essential for DNA biosynthesis. In addition, TS is an important cellular target for the fluoropyrimidine cytotoxic drugs that are widely used in the treatment of solid tumors. We have generated five monoclonal antibodies against human TS using a recombinant human TS enzyme. These antibodi...

We have evaluated for their potential use in the routine clinical laboratory enzyme-linked immunosorbent assays (ELISA) for human thyroglobulin antibodies (hTg-Ab) and microsomal antibodies (M-Ab). Results are expressed in terms of an "ELISA Index," based on comparison with a laboratory standard. The specificity of both ELISA assays is shown by dose-dependent inhibition of the hTg-Ab and M-Ab a...

cry genes encoding cry proteins toxic to lepidoptera, coleoptera and diptera species were studied in thirty seven b. thuringiensis strains isolated from twelve naturally infested heliothis armigera larvae. to further confirm the isolates, two groups of species-indicative biochemical tests were applied while discriminative biochemical tests being employed to figure out the repetitive strains. a ...

Mining activities are usually associated with environmental pollution and the infiltration of toxic heavy metals into waterways which could be absorbed by plants or used inhabitants. The aim this research was to determine effect mining on some biochemical parameters Opanda-Ugya inhabitants, Toto Local government Nigeria. Four hundred fifty (450) people were interviewed using questionnaires. Eig...

High throughput analyses in biochemical assays are gaining popularity in the post-genomic era. Multiple label-free detection methods are especially of interest, as they allow quantitative monitoring of biomolecular interactions. It is assumed that the sensor surface is stable to the surrounding medium while the biochemical processes are taking place. Using the Interferometric Reflectance Imagin...

In this paper, methods of sample preparation for potentiometric measurement of phenylalanine are presented. Basing on the spectrophotometric measurements of phenylalanine, the concentrations of reagents of the enzymatic reaction (10 mM L-Phe, 0,4 mM NAD, 2U L-PheDH) were determined. Then, the absorption spectrum of the reaction product, NADH, was monitored (maximum peak at 340 nm). The results ...

A hypothesis is presented that electronic and vibrational resonant energy states are important in biochemical reactions due to their coupling to hydrogen transfer reactions. The hypothesis is based upon (1) the involvement of hydrogen in most biochemical reactions, (2) the unique abiity of hydrogen to act as an energy trap for resonant energy and (3) demonstrations of the coupling of resonant e...

A 1-year longitudinal study was conducted in Magugu in Babati district, northern Tanzania to determine malaria vector population structure and malaria transmission indices. Mosquitoes were sampled using the Centre for Disease Control (CDC) light traps. A total of 110,357 adult female mosquitoes were collected. Anopheles gambiae s.1. accounted 25% of the total female mosquito collected. Relative...

Lysophospholipid acyltransferases (LPATs) incorporate fatty acyl chains into phospholipids via a CoA-dependent mechanism and are important in remodeling phospholipids to generate the molecular species of phospholipids found in cells. These enzymes use one lysophospholipid and one acyl-CoA ester as substrates. Traditional enzyme activity assays engage a single substrate pair, whereas in vivo mul...

BACKGROUND Anti Mullerian hormone (AMH) has previously been measured using a manual method, but a fully automated assay from Roche Diagnostics was recently introduced. The aim of this study was to compare the results from the AMH gen II ELISA and Elecsys Cobas AMH methods in a clinical setting to evaluate whether the assays achieve the goals of analytical performance. A prospective observationa...