This paper reports the development, characterization, and application of a separation-free, homogeneous, and simple to perform fluorescence polarization bioassay for paclitaxel. The bioassay is based on the binding interaction of paclitaxel to tubulin, the receptor protein on which this drug acts. The bioassay was carried out in a competitive format where the paclitaxel and a synthetic fluoresc...

An experimental tumor model system has been designed to permit quantitative study of the growth of a solid tumor and its métastasesand quantitative study of the response of this tumor and its métastasesto chemotherapy. The Lewis lung carcinoma was transplanted i.m., and tumor growth was followed by serial diameter measurements; these data were then converted to tumor weight by means of a dete...

A bioassay of technical-grade endosulfan for possible carcinogenicity was conducted using Osborne-Mendel rats and B6C3F1 mice. Endosulfan was administered in the feed, at either of two concentrations, to groups of 50 male and 50 female animals of each species. The time-weighted average high and low dietary concentrations of endosulfan were, respectively, 952 and 408 ppm for the male rats, and 4...

BACKGROUND Prolactin is a lactogenic hormone associated with breast cancer risk in prospective studies, which used immunoassays. The immunoassay captures multiple isoforms and may not fully reflect the biologic activity of prolactin relevant to breast carcinogenesis. METHODS We considered plasma bioactive prolactin levels measured by the Nb2 lymphoma cell bioassay, which is sensitive to the s...

PubChem's BioAssay database (https://pubchem.ncbi.nlm.nih.gov) has served as a public repository for small-molecule and RNAi screening data since 2004 providing open access of its data content to the community. PubChem accepts data submission from worldwide researchers at academia, industry and government agencies. PubChem also collaborates with other chemical biology database stakeholders with...

A laboratory bioassay was developed for testing tarnished plant bug populations for resistance development to the neonicotinoid insecticides imidacloprid and thiamethoxam. The bioassay allows for the determination of LC50 values by feeding known doses of the insecticides to adult tarnished plant bugs in a honey-water solution. Field populations of plant bugs from several locations in the mid-So...

-Peripheral-blood basophils were counted, in thick smears, in samples from patients with primary bronchial carcinoma, from patients in the same wards and from normal individuals. The median counts for patients with other chest conditions (15-5/4d) and bronchial-carcinoma patients free of tumour months to years after resection (16/1l) did not differ from those for normal individuals (19/1,u), bu...

BACKGROUND Experimental investigation of stroke, in particular the evaluation of therapeutic maneuvers, is difficult because even well-controlled experiments show considerable variability. Optimal use of resources requires efficient statistical analysis. SUMMARY OF REVIEW A body of experience in pharmacology that is directly applicable to stroke studies is reviewed. The general approach, thou...

an agar well diffusion bioassay method for determination of clarithromycin in human plasma, using micrococcus luteus atcc 9341 as the assay organism, was compared with a selective high-performance liquid chromatographic (hplc) method with uv detection. spiked plasma was used to prepare standard and control samples for both methods. the results of the bioassay analyses with spiked plasma samples...

The aim of the present study was to examine the resistance of PermaNet® 2.0 bed nets against repeated washing and environmental factors by using bioassay tests. After 5, 15 and 21 washings with detergents and by using bioassay tests, the resistance of 40 PermaNet® 2.0 bed nets was compared with that of 40 bed nets conventionally treated with one K-O tablet. To examine the long-term resistance, ...