Introduction: Maclura pomifera, is small deciduous tree which commonly called as Osage orange. It typically grows about 8 to 15 meters tall. pomifera secrete white latex material sticky in nature on damaging. The common name of this plant not related with citrus fruit family “orange” belongs mulberry Moraceae. Method: extract leaves was prepared by using ethanol a solvent. major groups phytoche...

BACKGROUND Monoamine oxidase-B (MAO-B) inhibitors are widely used in the treatment of Parkinson's disease. They increase vital monoamine neurotransmitters in the brain. However, there is a need for safer natural reversible MAO inhibitors with MAO-B selectivity. Our previous studies showed that Psoralea corylifolia seeds (PCS) extract contains compounds that inhibit monoamine oxidase-B. METHOD...

We evaluated the performance of the PG-Numeric (PGN; Isolab, Inc.) enzymatic assay for phosphatidylglycerol (PG) adapted for semi-automated analysis on the Cobas-Bio (Roche) centrifugal analyzer. We evaluated precision, linearity, and potential interference from endogenous glycerol. In addition, we compared the results of the PGN assay with those for two other PG assays. Amniostat-FLM (AFLM; Ha...

BACKGROUND The use of nanotechnologies for diagnostic applications shows great promise to meet the rigorous demands of the clinical laboratory for sensitivity and cost-effectiveness. New nanodiagnostic tools include quantum dots (QDs), gold nanoparticles, and cantilevers. QDs, which are the most promising nanostructures for diagnostic applications, are semiconductor nanocrystals characterized b...

We describe the development and validation of a labeled-hapten competitive immunoassay for determining total estrone in serum. For the hapten tracer we use the 6 alpha-biotinylated estrone derivative, 3-hydroxyestra-1,3,5(10)-trien-17-one 6 alpha-N-(epsilon-biotinyl)aminocaproamide (Bio-E1). A specific polyclonal rabbit anti-estrone antibody is indirectly bound via an immobilized donkey anti-ra...

INTRODUCTION In order to employ Nicotiana tabacum cells as a profitable natural bioreactor for production of bio-functional "Soluble human TRAIL" (ShTRAIL), endoplasmic reticulum (ER) targeted expression and innovative extraction procedures were exploited. METHODS At first, the ShTRAIL encoding gene was sub-cloned into designed H2 helper vector to equip it with potent TMV omega leader sequenc...