A basic lectin (pI approximately 10.0) was purified to homogeneity from the seeds of winged bean (Psophocarpus tetragonolobus) by affinity chromatography on Sepharose 6-aminocaproyl-D-galactosamine. The lectin agglutinated trypsinized rabbit erythrocytes and had a relative molecular mass of 58,000 consisting of two subunits of Mr 29,000. The lectin binds to N-dansylgalactosamine, leading to a 1...

During gestation the epidermis develops from a single layer of ectoderm into a layer of keratinocytes overlaid by a layer of periderm; this is followed by a progressive increase in the number of layers of keratinocytes, until finally the distinct granular and cornified layers characteristic of mature epidermis are formed. As part of our investigation into the function of the peanut lectin-bindi...

Angelika Preisfeld*, Gabriele Scholten-Beck, Hans Georg Ruppel Universität Bielefeld, Fakultät für Biologie, Postfach 100131, D-33501 Bielefeld, Bundesrepublik Deutschland Z. Naturforsch. 52c, 33-41 (1997); received September 20/0ctober 29, 1996 Deficiency, Euglena gracilis, Lectin Assay, Mucilage Biochemical and ultrastructural examinations on the pellicle of autotrophically grown Eu­ glena gr...

BACKGROUND Pathways coordinated by innate pattern recognition receptors like mannose-binding lectin (MBL) and nucleotide-binding oligomerization domain 2 (NOD2) are among the first immune responses to Staphylococcus aureus (S. aureus) bloodstream infections (BSI) in animal models, but human data are limited. Here, we investigated the role of MBL deficiency and NOD2 mutations in the predispositi...

Equilibrium dialysis studies on the binding of the Dolichos biflorus lectin with [14C]methyl alpha-D-GalNAc showed that the lectin has two combining sites/molecule and an intrinsic association constant at 3 degrees C of 4.2 X 10(3) liters mol-1. Binding studies on individual fractions (II, IV, and VII) of the lectin that differ in their chromatographic properties on concanavalin A-Sepharose gav...

The binding of 125I-asialoorosomucoid and the isolated rabbit hepatic lectin were studied at 0 degrees C. The steady state binding data were analyzed both by a direct curve-fitting procedure that utilizes nonlinear least squares regression analysis, and by conventional Scatchard plot analysis. Two classes of binding sites, present in approximately equal concentrations, were detected by both ana...

The isolation of lectins by affinity chromatography with crosslinked hemicelluloses has been a common practice because the variety glycosides that they present, improving different kinds lectins, such as galactose ligands.Lectins for carbohydrates is so specific simple configuration chiral carbon can affect affinity, and there are more related to alfa-galactosidic than beta-galactosidic residue...

The roles of selected amino acid residues of human 14-kDa beta-galactoside-binding lectin were studied by site-directed mutagenesis. Ten mutant lectin proteins were produced, in each of which one of the residues regarded as possibly related to the stability of the lectin (6 cysteine residues) or one of those highly conserved in the vertebrate beta-galactoside-binding lectin family (Asn46, Trp68...