human serum albumin (hsa) is the most abundant protein constituent of blood plasma. this protein consists of a single polypeptide chain of 585 amino acid residues, which has many important physiological functions. hsa can bind and carry many drugs, including anticoagulants, tranquilizers, and general anesthetics. some technique such as: fluorescence spectroscopy, three-dimensional fluorescence ...

the interaction between propranolol (prop) and human serum albumin (hsa) was studied in the presence of dimethyl methylphosphonate (dmmp). dmmp is usually considered as a simulant for chemical warfare agents (cwas). for this purpose fluorescence quenching, resonance light scattering (rls), synchronous, three-dimensional fluorescence spectroscopy and molecular dynamics (md) simulation were emplo...

Kinetic and thermodynamic studies have been made on the effect of the inosine product on the activity of adenosine deaminase in a 50 mM sodium phosphate buffer, pH 7.5, at 27 degrees C using UV spectrophotometry and isothermal titration calorimetry (ITC). A competitive inhibition was observed for inosine as a product of the enzymatic reaction. A graphical-fitting method was used for determinati...

PEPPSI type Pd complexes have wide applications in recent years. Serum albumin carries very important molecules such as oleic and linoleic acids, nitric oxide, vitamin B6, thyroid so do many drugs. In this study, a novel NHC molecule its Pd-PEPPSI complex were synthesized characterized. Both the ligand optimized by DFT-based calculation methods. The binding properties of with HSA analyzed molec...

The initiation of transcription involves three steps: binding, opening, and escape of RNA polymerase. To model these steps in the simplest way we will treat opening and escape as a single reaction with forward reaction rate k determined by the regulatory proteins and their interaction with the DNA. Binding will be treated as a reversible reaction with an equilibrium constant KB. Using mathemati...