Electron paramagnetic resonance spectra of M&II) bound to pyruvate kinase and its substrate and inhibitor complexes are examined in solution. The binary complex of Mn(I1) with the enzyme gives a spectrum which indicates that the electronic symmetry of the bound cation is only moderately distorted upon liganding to the protein. The spectrum of the binary enzyme-Mn(I1) complex is unaltered upon f...

Ab initio MP2/aug’-cc-pVTZ calculations have been performed to determine selected stretching frequencies and chemical shieldings for ternary complexes N-base:PH2F:BeX2 the corresponding binary complexes, with NH3, H2C=NH, HCN as nitrogen bases H, F, Cl substituents X. Be-F P-F depend on distances, respectively, while P F N-P respectively. The graph of versus distance bears a remarkable resembla...

MOTIVATION A growing body of research has concentrated on the identification and definition of conserved sequence motifs. It is widely recognized that these conserved sequence and structural units often mediate protein functions and interactions. The continuing advancements in high-throughput experiments necessitate the development of computational methods to critically assess the results. In t...

A spin-labeled derivative of rabbit muscle creatine kinase has been prepared by reaction of the essential sulfhydryl group at the active sites with a stoichiometric amount of the nitroxide radical N-( I-oxyl-2,2,5,5-tetramethyl-d-pyrrolidinyl) iodoacetamide. The spin-labeled enzyme retains the ability to bind nucleotide substrates although it lacks the phosphotransferase and ATPase activities o...

The crystal structure of the phosphorylating glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from Bacillus stearothermophilus was solved in complex with its cofactor, NAD, and its physiological substrate, D-glyceraldehyde 3-phosphate (D-G3P). To isolate a stable ternary complex, the nucleophilic residue of the active site, Cys(149), was substituted with alanine or serine. The C149A and C149S G...

Thermoresponsive immunonephelometry was achieved with biotinylated poly(acrylate) and thermoresponsive gold nanocomposites composed of 13-nm gold nanoparticles and thermoresponsive polymers containing triethylenetetramine and biotin groups. The avidin-biotin interaction was used to model an immunoreaction in order to demonstrate thermoresponsive immunonephelometry. In the absence of avidin, pos...

Expression of viral oncoproteins results in the loss of cell cycle checkpoint control and the accumulation of chromosomal abnormalities. Expression of both human papillomavirus type 16 oncoproteins, E6 and E7, in normal human fibroblasts completely dissociates p21 and proliferating cell nuclear antigen from the quarternary cyclin-cyclin-dependent kinase (CDK) complexes present in normal cells, ...

Recently a theoretical analysis of PCR efficiency has been published by Booth et al., (2010). The PCR yield is the product of three efficiencies: (i) the annealing efficiency is the fraction of templates that form binary complexes with primers during annealing, (ii)the polymerase binding efficiency is the fraction of binary complexes that bind to polymerase to form ternary complexes and (iii)th...

The geometry of the binary and ternary complexes of two black-eyed pea inhibitors with trypsin and chymotrypsin has been established by distance measurements using the technique of singlet-singlet energy transfer. Triangulation of measured distances in the ternary double-headed complex of the trypsin-chymotrypsin inhibitor (BEPCI) with trypsin and chymotrypsin limits the possible structural mod...

We use Grayson’s binary multicomplex presentation of algebraic K-theory to give a new construction of exterior power operations on the higher K-groups of a (quasi-compact) scheme. We show that these operations satisfy the axioms of a λ-ring, including the product and composition laws. To prove the composition law we show that the Grothendieck group of the exact category of integral polynomial f...