two independent experiments were conducted with broilers to determine the effect of threonine and vitamin a on immune system. in first experiment, dietary treatments contained 0.8%, 0.87%, 0.94 % and 1.01 % total threonine. in second experiment, diets were as follows: basal diet without vitamin a; basal diet plus 1500 iu/kg vitamin a; basal diet plus 6250 iu/kg vitamin a; basal diet plus 11000 ...

Methods We present 8 children with suspected hypersensitivity reaction after parenteral MP (6 children), DX inhalation (1 child) and inhafluticasone propionate (FL) (1 child). Prick and intradermal skin testing with MP, HC, DX and FL were performed. In case of negative skin tests provocation tests were performed. Basophil activation test (flow cytometry analyses of basophil CD63 surface express...

Jones-Mote reactions are delayed, erythematous, and mildly indurated cutaneous reactions originally described in humans sensitized by skin injection of heterologous proteins. Similar reactions in guinea pigs contain many basophils and are called cutaneous basophil hypersensitivity. In contrast, guinea pigs immunized with mycobacterial adjuvants have classical tuberculin-type delayed hypersensit...

BACKGROUND Papular urticaria by flea bite (PUFB) is a chronic inflammatory disease in children. The aim of this study was to assess the functional activity of IgE to protein fractions from flea body extract, through basophil degranulation in PUFB patients and controls. METHODS Basophil degranulation, measured by overexpression of CD63 surface molecules, was evaluated by flow cytometry in samp...

BACKGROUND A major problem of venom-specific immunotherapy (VIT) is the absence of reliable parameters for deciding treatment discontinuation. AIM OF THE STUDY Intracutaneous tests (ICTs), the basophil activation test (BAT), specific IgEs (sIgEs) and blocking factor (BF) activity were measured during VIT. We made an evaluation by means of a protective index (PI) including ICT, BAT and BF valu...

Recent experimental studies have shown that the basophil is critical for a subtype of anaphylaxis or IgEmediated very late-phase skin inflammation.1 Motility and activation of basophils are known to be regulated by various endogenous and or exogenous substances. The panel of known such basophil-directed molecules is expanding. One important candidate may be geranylgeranyl compounds, which were ...