نتایج جستجو برای: bacterial viability

تعداد نتایج: 268133  

Journal: :Langmuir : the ACS journal of surfaces and colloids 2009
Zhiyong Suo Recep Avci Muhammedin Deliorman Xinghong Yang David W Pascual

A bacterial cell wall is a highly dynamic multilayer structure interfacing the cytoplasm to the outside environment. It supports a multitude of chemical and biological processes necessary for life. It is therefore postulated that damage to the structure of bacterial cell wall would threaten cell integrity and result in cell death. We tested this hypothesis by repeatedly puncturing the cell wall...

Journal: :Gene 2009
Xiongying Cheng Guozhen Liu Gongyin Ye Haijiao Wang Xiaojing Shen Kechun Wu Jiahua Xie Illimar Altosaar

A novel method for cloning of genes coding for cytotoxic molecules based on a cell viability assay is described. The working hypothesis is that expression of DNA sequences coding for cytotoxic molecules in bacterial cells will lead to cell death or impairment, and the isolation of the impaired or dead cells could lead to identification of DNA sequences responsible for debilitating the host cell...

2015

The filter disc is used as base to support the spores inside the tiny holes. The filter paper discs are dipped into the medium after the spore phase growth of microorganisms. The whatman filter wet discs are dried at 55-60°C till they are completely dry. The viability of discs stored at room temperature and -20°C were checked at 3, 4, 6 and 2 month intervals. This is cost effective technique. I...

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