The present work describes the effects of winter and spring genotypes of rapeseed (PF7045/91, Okapi and Opera), explants (cotyledons and hypocotyls), light, different kinds and concentrations of auxins [Naphthylacetic acid (NAA) and 2, 4dichlorophenoxyacetic acid (2, 4-D)], presence of cytokinin [benzyladenine (BA)] and different concentrations of micro and macro elements (MS and 1/2MS) on call...

INTRODUCTION Auxins are hormones that regulate plant growth and development. To accurately quantify the low levels of auxins present in plant and soil samples, sensitive detection methods are needed. In this study, the design and construction of two different whole cell auxin bioassays is illustrated. Both use the auxin responsive element HpaA as an input module but differ in output module. The...

Somatic embryogenesis (SE) is a powerful tool for plant genetic improvement when used in combination with traditional agricultural techniques, and it is also an important technique to understand the different processes that occur during the development of plant embryogenesis. SE onset depends on a complex network of interactions among plant growth regulators, mainly auxins and cytokinins, durin...

BACKGROUND Plant body plans arise by the activity of meristematic growing tips during development and radiated independently in the gametophyte (n) and sporophyte (2n) stages of the life cycle during evolution. Although auxin and its intercellular transport by PIN family efflux carriers are primary regulators of sporophytic shoot development in flowering plants, the extent of conservation in PI...

Micropropagation of Asclepias curassavica L. a medicinal plant valued for its medicinal properties has been carried out using two different plant culture media, Murashige and Skoog (MS) and L2. L2 medium proved to be superior to MS medium in terms of shoot multiplication and shoot length. Nodal explants showed enhanced organogenic response than shoot tip explants. Basal nodal explants produced ...

Auxins, mainly indole-3-acetic acid (IAA), play a critical role in numerous plant growth processes, including embryo development, root and flower development, vascular differentiation, stem elongation, apical dominance, and tropic responses. Charles Darwin and his son Francis performed some of the earliest work relating to the effect of auxin on plant growth with their experiments on phototropi...

The low yield and high market price of the pharmaceutically important alkaloids of Catharanthus roseus (L.) G. Don viz. vincristine, vinblastine and ajmalicine have created interest in improved alternative routes for their production such as using cell and tissue culture. The callus developed on Murashige and Skoog (MS) media supplemented with different concentrations of auxins and cytokinins w...

Quantitative Structure-Activity Relationship (QSAR) study on 22 1H-indole-3-acetic acid derivatives with auxin activity was performed by means of Principal Component Analysis (PCA), Hierarchical Cluster Analysis (HCA), Partial Least Squares Regression (PLS) and Multiple Linear Regression (MLR). Molecular geometry of the auxins was optimized at MMFF94 and ab initio B3LYP 6-31G** levels. Modeling...

Auxinic-like compounds have been widely used as weed control agents. Over the years, the modes of action of auxinic herbicides have been elucidated, but most studies thus far have focused on their effects on later stages of plant growth. Here, we show that some select auxins and auxiniclike herbicides trigger a rapid elevation in root cytosolic calcium levels within seconds of application. Arab...

Auxin plays a key role in regulation of almost all processes of plant growth and development. Different physiological processes are regulated by different ranges of auxin concentrations; however, the underlying mechanisms creating these differences are largely unknown. The first step of auxin signaling is auxin-dependent interaction of an auxin receptor with transcriptional co-repressors (Aux/I...