Sixty intact, non-carious and unrestored teeth extracted due to periodontal disease were used to determine the most effective method of sterilisation. The teeth were divided into six groups, each containing 10 teeth. Group 1 teeth were immersed in 10% formalin for seven days, group 2 teeth were immersed in 3% hydrogen peroxide for seven days, group 3 teeth were immersed in 2.6% sodium hypochlor...

AIM To evaluate the effects of repeated autoclaving on torsional strength of two nickel-titanium (NiTi) rotary endodontic files: Twisted Files (SybronEndo, Orange, CA, USA) and GT Series X files (Dentsply Tulsa Dental Specialties, Tulsa, OK, USA). METHODOLOGY Four groups of 20 GT Series X (size 20, .06 taper) and four groups of 20 Twisted Files (size 25, .06 taper) were subjected to 0, 1, 3 o...

A new thermogelling chitosan-glycerophosphate system has been recently proposed for biomedical applications such as drug and cell delivery. The objectives of this work were to characterize the effect of steam sterilization on the in vitro and in vivo end performances of the gel and to develop a filtration-based method to assess its sterility. Autoclaving 2% (w/v) chitosan solutions for as short...

Abstract To our knowledge, there are few studies on the association between carbohydrate molecular structure spectral profiles of barley grain and ruminant-relevant nutritional characteristics. This study aimed to associate processing-induced changes in with profiles. The heat processing methods included: dry roasting, autoclaving, microwave irradiation. were determined which included chemical ...

The synergistic and inhibitory interactions observed between Fibrobacter succinogenes A3c, Prevotella ruminicola H2b, and Ruminococcus flavefaciens B34b in the digestion of forage cellulose were studied in detail. Orchardgrass and alfalfa hays, both at two maturity stages, were used as substrates. Sequential inoculation procedures were developed whereby a second inoculation was made after the i...

Mycobacterium avium subsp. paratuberculosis (MAP) purified protein derivatives(PPDs) are an immunologic reagent prepared from the cultured filtrate of MAP ATCC strain19698. PPD was produced using MAP ATCC Strain 19698 in Povitsky bottles and Erlenmeyerflasks. Traditional production consisted of floating culture incubation at 37C, live organisminactivation by autoclaving 30 minut...

The stability of a formulated heparin was examined during its sterilization by autoclaving. A new method to follow loss in heparin binding to the serine protease inhibitor, antithrombin III, and the serine protease, thrombin, was developed using a surface plasmon resonance competitive binding assay. This loss in binding affinity correlated well with loss in antifactor IIa (thrombin) activity as...