The extraction of xylanase was performed using an aqueous two-phase system (ATPS) based on polyethylene glycol (PEG1540) and various salts. Preliminary studies in a batch extractor showed that the highest efficiency, E = 79.63 ± 5.21%, purification factor, PF 1.26 0.25, were obtained with sodium citrate dihydrate-H2O-PEG1540-based ATPS for time 10 min. process optimized experimental Box-Behnken...

Abstract Background Bacteriocins are generally defined as ribosomally synthesized peptides, which produced by lactic acid bacteria (LAB) that affect the growth of related or unrelated microorganisms. Conventionally, extracted bacteriocins purified precipitation, where ammonium sulphate is added to precipitate out protein from solution. Main text To achieve high purity bacteriocins, a combinatio...

Aqueous Two Phase System (ATPS) or liquid-liquid extraction is used in biotechnology to recover valuable compounds from raw sources. In Two-Phase Systems, many factors influence the Partition coefficient, K, (which ratio of protein concentration top phase that bottom phase) and Recovery percentage (Rec%). this research, two systems ATPS were used: first, polyethylene glycol (PEG) 4000/Sodium ci...

BACKGROUND Microalgae can synthesize starch with productivity higher than conventional terrestrial crops, without the need for arable land. However, little is known about processes to extract from microalgae. Here, a biorefinery process described including microalgal cell disruption followed by extraction of and pigments aqueous two-phase system (ATPS) using choline chloride polypropylene glyco...

The future challenges in bioprocessing include developing new downstream processes for the purification and manufacture of the protein based medicines of the future to relieve the predicted bottleneck being produced by increasingly high titres from fermentation processes. This review looks at the recent developments in centrifugal liquid-liquid partition chromatography using aqueous two-phase s...