The atomic force microscope (AFM) can be used to visualize and to manipulate biological material with relative case and high resolution. This study was carried out to investigate whether probe sets, specific for subregions of the human genome and useful for the painting of chromosome bands, can be established by PCR amplification of AFM-dissected chromosome regions. Compared to standard microdi...

Mapping the force on plasmid DNA molecules with the atomic force microscope (AFM) showed an increased adhesion force between a silicon nitride tip and DNA strands in a mildly acidic solution. With this adhesion force, the plasmid DNA could be stretched off the mica surface, and the silicon nitride tip occasionally picked up one or several molecules of plasmid DNA from the mica surface. We have ...

Atomic force microscope (AFM) images often contain distortions caused by the convolution of the tip and sample surface. The offered numerical deconvolution, with the use of an inverted tip located at a constant height from the surface, noticeably differs from methods previously published and is essentially simple for a concrete practical realization. Nanometer-sized spheres were successfully us...

The microstructure morphologies of copper bonded wafers were examined by means of transmission electron microscopy (TEM) and atomic force microscope (AFM). Morphologies of non-distinct, zigzag and distinct interfaces in the bonded layer are observed. A strong relationship between the roughness of surfaces and the individual steps in bonding initiation was found. We propose three different mecha...

The problem of assessing the quality combining component images formed in two synchronous channels an atomic force microscope (AFM images), is considered. A computationally simple metric for AFM images, based on local correlation coefficients proposed, taking into account contribution each to resulting combined image and between images. It shown that provides a higher accuracy combination estim...

Nanomachining of thin polymer resist films with an atomic force microscope (AFM) is a promising route for the fabrication of nanoscale devices. In order to enhance the controllability of the nanomachining process an in-plane acoustic wave is coupled to the sample support. This enhances the intermittent force exerted by the AFM tip. The lateral resolution reached by this method is only limited b...

Sub diffraction limited infrared absorption imaging of hemoglobin was performed by coupling IR optics with an atomic force microscope. Comparisons between the AFM topography and IR absorption images of micron sized hemoglobin features are presented, along with nanoscale IR spectroscopic analysis of the metalloprotein.

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We use an atomic force microscope (AFM) to manipulate graphene films on a nanoscopic length scale. By means of local anodic oxidation with an AFM we are able to structure isolating trenches into single-layer and few-layer graphene flakes, opening the possibility of tabletop graphene based device fabrication. Trench sizes of less than 30 nm in width are attainable with this technique. Besides ox...

Atomic force microscope (AFM) has been used incrementally over the last decade in cell biology. Beyond its usefulness in high resolution imaging, AFM also has unique capabilities for probing the viscoelastic properties of living cells in culture and, even more, mapping the spatial distribution of cell mechanical properties, providing thus an indirect indicator of the structure and function of t...