For L-aspartic acid biosynthesis, high production cells of Escherichia coli mutant B-715 and P1 were immobilized in chitosan gel using a technique developed in our laboratory. The immobilization process reduced initial activity of the intact cells, however, the biocatalyst produced was very stabile for long-term use in multi-repeated batch or continuous processes. Temperature influence on the c...

Using molecular dynamics simulations and nuclear magnetic resonance techniques we explored the conformational preferences of linear and chiral analogs of acetyl-arginyl-glycyl-aspartic acid (Ac-RGD) and acetyl-arginyl--Alanyl-aspartic acid (Ac-RXD). Using tinker molecular modeling program and 5nano second molecular dynamics simulations in water/dimethyl sulphoxide to mimic the varying environm...

Cyanide disappears from a medium supporting actively growing cells of Bacillus megaterium, a bacterium isolated from a cyanide enrichment medium containing Fargo clay soil. Whole cells of this organism convert K14CN to asparagine-*4C, aspartic acid-14C and 14C02. Besides cyanide, serine l4C and /3-cyanoalanine-14C are effectively converted by whole cells of this organism to asparagine-14C. An a...

Halophilic organisms have adapted to survive in high salt environments, where mesophilic organisms would perish. One of the biggest challenges faced by halophilic proteins is the ability to maintain both the structure and function at molar concentrations of salt. A distinct adaptation of halophilic proteins, compared to mesophilic homologues, is the abundance of aspartic acid on the protein sur...

In a previous study we reported low values of taurine and aspartic acid in the CSF of patients with Friedreich's ataxia, when the results were compared to the literature. Further studies have revealed that unforetold difficulties with the advertised methodology of sequential multi-sample amino acid analysis were responsible for low values in the determination of these two amino acids in the sma...

Both water soluble lysine- and aspartic acid-based amino acid ionic liquids (AAILs) were mixed to prepare polar AAILs showing phase separation with water.

15 Roberts, R. B., P. H. Abelson, D. B. Cowie, E. T. Bolton, and R. J. Britten, Carnegie Institution of Washington Publication 607, Washington, D. C. (1955). 16 The rather low values for threonine and lysine obtained with both labeled aspartic acids in Lemna probably are significant and may indicate a "channeling" of aspartic acid, i.e., a preferential utilization of aspartic acid for incorpora...

Although biotin is required for the synthesis of the inducible malic enzyme in Lactobacillus arabinosus 17-5 (I), partially purified preparations of the enzyme do not contain biotin (a), and avidin does not affect the activity of the enzyme (3). The formation of another enzyme, ornithine transcarbamylase, in iStreptococcus Zactis 8039 is also diminished by a biotin deficiency (4), but purified ...

Two variant glycopeptide constituents of an hepatic membrane protein responsible for the clearance of circulating asialoglycoproteins have been isolated and characterized. Their relative abundance has been estimated to be 14 and 2 mol, respectively, per mol of intact protein. The larger component, glycopeptide I, is comprised of sialic acid, galactose, mannose, glucosamine, and aspartic acid in...

AIM The quest to identify an accurate method of age estimation, had lead to the evaluation of aspartic acid racemisation in hard tissues of the human remains using high performance liquid chromatography (HPLC). Our study is aimed at the applicability of the racemisation technique to use dentin as the sample to estimate the age in South Indian sub-population. MATERIALS AND METHODS Thirty-six n...