Aspartate aminotransferase from Sulfolobus solfataricus (SsAspAT) is an extremely thermophilic and thermostable dimeric enzyme which retains its structure and reaches maximal activity at 100 degrees C. The structural stability of this protein was investigated by coupling isothermally and thermally induced denaturation studies to molecular modeling. Gel filtration analysis indicated that SsAspAT...

The intra-subject correlations of three clinically meaningful combinations of serum constituents--(a) potassium, calcium, and albumin; (b) urea, creatinine, and uric acid; and (c) aspartate aminotransferase, lactate dehydrogenase, and alkaline phosphatase--were studied in 11 healthy men. Duplicate serum samples were obtained at 800 h, 1100 h, and 1400 h on five different days. All assays were p...

Cytosolic and mitochondrial aspartate aminotransferase cDNAs were cloned from a lambda gt11 rat liver cDNA library. The complete coding sequence and the 3' non-coding sequence of the cytosolic isozyme mRNA were obtained from two overlapping cDNA clones. Partial sequences of the mitochondrial enzyme cDNAs were found to be identical to the recently published complete sequence (Mattingly, J. R., J...

Aspartate aminotransferases have been cloned and expressed from Crithidia fasciculata, Trypanosoma brucei brucei, Giardia intestinalis, and Plasmodium falciparum and have been found to play a role in the final step of methionine regeneration from methylthioadenosine. All five enzymes contain sequence motifs consistent with membership in the Ia subfamily of aminotransferases; the crithidial and ...

Dexamethasone, insulin, and serum induce an additive increase in the activity of tyrosine aminotransferase (L-tyrosine : Z-oxoglutarate aminotransferase, EC 2.6.1.5) in hepatoma cells in tissue culture. By the criteria of immunotitration, heat stability, and electrophoresis on polyacrylamide gels, we have shown previously that all three effecters increase the cellular concentration of the same ...

BACKGROUND Liver fibrosis which is a common complication of chronic hepatitis B infection is rarely diagnosed in low-resource countries due to limited capacity to perform biopsy studies. Data on the utilization of noninvasive techniques which are feasible for diagnosis of liver fibrosis in these settings among HIV-infected patients is scarce. The objective of this study was to establish the mag...

In this study, gene encoding Aspartate aminotransferase was cloned from Arabidopsis thaliana by RT-PCR, named F23N and its bioinformatics analysis was performed. It was found that there is one pyridoxal-phosphate attachment site at 250-270 (SYAKnmGLyGERIG) and other domains, furthermore, the transmembrane helices was not found in F23N, suggesting that F23N encoding product might not belong to m...