Crystalline glutaminase-asparaginase with antitumor activity was prepared from an Achromobacferaceae soil isolate organism. This enzyme has L-glutaminase and L-asparaginase activity in a ratio of 1.2:1. The purification procedure provides an over-all yield of 40 to 60% from crude cell-free extract to homogeneous glutaminase-asparaginase and is adaptable to large scale isolation of the enzyme. G...

L-Asparaginase (E.C. 3.5.1.1) is used as an anti-neoplastic drug in the treatment of acute lymphoblastic leukemia. L-Asparaginase from Pseudomonas fluorescens was cloned and overexpressed in E. coli BL21. The Enzyme was found to be a Fusion protein-asparaginase complex which was given a lysozyme treatment and sonication, and then was purified in a Sepharose 6B column. The enzymatic properties o...

Asparaginase is an effective treatment for patients with acute lymphocytic leukemia (ALL). Unfortunately, asparaginase therapy is associated with a high incidence of hypersensitivity reactions (up to 73%), including life-threatening anaphylaxis, and its half-life of approximately 20 hours necessitates daily administration. Pegaspargase, a modification of L-asparaginase, has a longer half-life (...

L-Asparaginase was added to vincristine and prednisone for induction of first remission in 815 children with acute lymphocytic or acute undifferentiated leukemia. This combination resulted in an overall remission rate of 93%. The addition of L-asparaginse to the standard induction regimen using prednisone and vincristine did not significantly increase the morbidity or mortality rate during the ...

L-Asparaginase, in the dose of greater than or equal to 6000 IU/sq m three times weekly, was demonstrated to be an effective agent in reinduction of remissions in childhood leukemia. Four hundred thirteen children with acute lymphocytic leukemia were treated with L-asparaginase. Doses i.m. ranged from 300 to 12,000 IU/sq m. None of the patients had received prior asparaginase therapy. 6-Mercapt...

The enzyme L-asparaginase has been proved to be efficient in treatment of Acute Lymphoblastic Leukemia [ALL] and Lymphosarcomas. The present paper discusses the studies carried out for the production of this enzyme from bacillus species. By study obtained optimization of medium, solid state fermentation by using cotton cake and wheat bran and submerged fermentation using distillery waste. a M2 ...

The objective of this study was to isolate halophilic bacteria with the ability to produce intracellular or extracellular L-asparaginase. A total number of 120 halophilic bacteria were isolated from 17 different saline habitats of Iran including salt lakes, wetlands, brine springs and deserts. Among these, 68 were able to grow in the presence of 1.5 M NaCl and 52 demonstrated the ability to gro...

This paper describes optimization method that combines the Plackett-Burman design, a factorial design and the response surface method, which were used to optimize the medium for the production of L-asparaginase by Serratia marcescens SB08. Four medium factors, from out of 11 medium factors, were screened by Plackett-Burman design experiments and subsequent optimization process to find out the o...

Expression of the two Bacillus subtilis genes encoding L-asparaginase is controlled by independent regulatory factors. The ansZ gene (formerly yccC) was shown by mutational analysis to encode a functional L-asparaginase, the expression of which is activated during nitrogen-limited growth by the TnrA transcription factor. Gel mobility shift and DNase I footprinting experiments indicate that TnrA...

Endophytic fungi generally reside asymptomatically in the tissues of higher plants and generally are source of original amidase group of enzymes. Asparaginase is an important anticancer enzyme, endophytic fungi Eurotium Sp. was isolated from rhizomes of Curcuma longa and evaluated for asparaginase production by qualitative plate assay on modified Czapex dox’s agar and quantitative analysis by N...