Nowadays, polymer nanofibers have gained attention due to remarkable characteristics such as high porosity and large surface area to volume ratio. Among their fabrication methods, electrospinning technique has been attracted as a simple and reproducible approach. It is a versatile, simple and cost-effective technique for the production of continuous nanofibers with acceptable characteristics su...

A method is described which results in a 2750-fold purification of hydrogenase from Chlamydomonas reinhardtii, yielding a preparation which is approximately 40% pure. With a saturating amount of ferredoxin as the electron mediator, the specific activity of pure enzyme was calculated to be 1800 micromoles H(2) produced per milligram protein per minute. The molecular weight was determined to be 4...

A cytochrome P450 (CYP) enzyme, 3'-daidzein hydroxylase, CYP105D7 (3'-DH), responsible for daidzein hydroxylation at the 3'-position, was recently reported. CYP105D7 (3'-DH) is a class I type of CYP that requires electrons provided through electron transfer proteins such as ferredoxin and ferredoxin reductase. Presently, we constructed an artificial CYP in order to develop a reaction host for t...

An artificial retroaldolase was developed using a 43-amino-acid-long miniprotein derived from the C-terminal domain of MvaT protein. A series rounds rational optimization activity performed, included grafting active motif in cleft miniprotein, engineering site structure, and modifying charge distribution on surface miniprotein. It proved that miniproteins this size can be mutated to obtain an e...

Adv. Mater. 2021, 33, 2006363 DOI: 10.1002/adma.202006363 The above article, published online on December 6, 2020, in Wiley Online Library (wileyonlinelibrary.com), has been retracted by agreement between the authors, journal Editor Chief Jos Lenders, and Wiley-VCH GmbH. authors asked to retract this article as subsequent experiments revealed that conclusions of manuscript are invalid. generati...

Artificial designer minicellulosomes comprise a chimeric scaffoldin that displays an optional cellulose-binding module (CBM) and bacterial cohesins from divergent species which bind strongly to enzymes engineered to bear complementary dockerins. Incorporation of cellulosomal cellulases from Clostridium cellulolyticum into minicellulosomes leads to artificial complexes with enhanced activity on ...

Typha latifolia (cattail) forms natural stands in the transition zone of artificial flotation and mine tailings ponds is contaminated with extremely high concentrations metals. We assessed absorption capacity plant, metal transfer to leaves, effects elevatedmetal on antioxidant enzyme activities. Soil acidity, pseudo-total available content substrate, plants were examined. The elevated activiti...

through the last three decades different seismological and engineering approaches for the generation of artificial earthquakes have been proposed. selection of an appropriate method for the generation of applicable artificial earthquake accelerograms (aeas) has been a challenging subject in the time history analysis of the structures in the case of the absence of sufficient recorded accelerogra...

An extracellular H(inf2)O(inf2)-requiring Remazol brilliant blue R (RBBR) decolorizing enzymatic activity was found in the culture medium of Pleurotus ostreatus. The enzymatic activity was maximally obtained in idiophase, and the optimum C/N ratio was 24. High C/N ratios repressed the enzymatic activity, and addition of veratryl alcohol had no effect on the production of enzyme. The enzyme was ...

Fibrinolytic enzymes have nowadays found important place in the medical field. Only few studies had reported the fibrinolytic enzyme production from actinomycetes. So the microbial fibrinolytic enzymes, especially those from actinomycetes, have the potential to be developed as drugs to prevent cardiovascular diseases. Actinomycetes were producing strong enzymes with no side effects, non pathoge...