Background. This study investigates the viable persistence of avian influenza viruses (AIVs) in various types of artificially frozen environmental water and evaluates the feasibility of similar occurrence taking place in nature, and allowing for prolonged abiotic virus survival, with subsequent biotic viral recirculation. Methods. Fresh, brackish, and salty water, taken in Japan from aquatic bi...

Analysis of environmental DNA (eDNA) enables the detection of species of interest from water and soil samples, typically using species-specific PCR. Here, we describe a method to characterize the biodiversity of a given environment by amplifying eDNA using primer pairs targeting a wide range of taxa and high-throughput sequencing for species identification. We tested this approach on 91 water s...

Flexible, or soft-shelled, eggs are almost unknown in the fossil record, leaving large gaps in our knowledge of the reproductive biology of many tetrapod clades. Here, we report two flexible-shelled eggs of the hyphalosaurid choristodere Hyphalosaurus baitaigouensis from the Early Cretaceous of China, one containing an embryo and the second associated with a neonate. Choristoderes are an enigma...

H6 subtype influenza viruses are commonly isolated from wild aquatic birds. However, limited information is available regarding H6 influenza virus isolated from chickens. We compared the viral genome segment between A/chicken/Hong Kong/W312/97 (H6N1), which was able to grow in chicken trachea, and A/duck/Shantou/5540/01 (H6N2), which was isolated from wild aquatic duck, to explore the factors f...

New carbon and nitrogen stable isotope values for human remains dating to the mid-Upper Paleolithic in Europe indicate significant amounts of aquatic (fish, mollusks, and/or birds) foods in some of their diets. Most of this evidence points to exploitation of inland freshwater aquatic resources in particular. By contrast, European Neanderthal collagen carbon and nitrogen stable isotope values do...

Today, interest continues to grow in the potential aquatic-toterrestrial transport of contaminants through movement of insects, birds, reptiles, and other organisms or via the energetic pathways that quintessentially link aquatic and terrestrial ecosystems. John Muir. Henry David Thoreau. Theodore Roosevelt. Rachel Carson. Preservationists, conservationists, politicians, scientists, authors, an...

The evolutionary diversi®cation of living organisms is a central research theme in evolutionary ecology, and yet it remains dicult to infer the action of evolutionary processes from patterns in the distribution of rates of diversi®cation among related taxa. Using data from helminth parasite communities in 76 species of birds and 114 species of mammals, the in ̄uence of four factors that may eit...