conclusions: e. coli herb-resistance plasmid can replicate and be expressed in s. aureus. results: the identified 45 kb herb-resistance plasmid could be transferred from e. coli cp9 isolates to e. coli dh5α. as a consequence e. coli dh5α transconjugant mic increased from 0.0125 g/ml to 0.25 g/ml. the plasmid was easily transferred from e. coli cp9 strain to s. aureus rn450rf with a mean transfe...

antisense oligodeoxynucleotides (oligos) are the tools that bind to complemen¬tary sequence of targeted mrna and block specifically protein translation. in the present study, a novel 20 mer oligo as an antisense for human il-13 is introduced. this oligo is designed according to the il-13 mrna coding region and synthesized in two hplc purified and fitc conjugated forms. fluorescence oligo cell u...

Currently there is much excitement in certain quarters about antisense oligonucleotides, both as the drugs of the future for treatment of cancer and viral infections, most notably AIDS, and also as genetic tools for generating mutant phenocopies without genomic mutation in investigations of mammalian gene function. The extent of this excitement is reflected in the proliferation of antisense bio...

background: pteridine metabolic pathway is unusual features of leishmania , which is necessary for the growth of parasite. leishmania has evolved a complex and versatile pteridine salvage network which has the ability of scavenging a wide area of the conjugated and unconjugated pteridines espe-cially folate and biopterin. in this study, we focus on the inhibition of ptr1 gene expression. method...

background: echinococcosis or hydatidosis is a chronic, zoonotic worldwide infection that occurs by the larval stages of taeniid cestodes of the genus echinococcus . iran is known as endemic region for this infection in the world. vaccination has been considered as a good prevention method for this disease. recombinant vaccines containing eg95 protein, against e. granulosus , has shown a high d...

Objective(s): The use of antisense oligonucleotides (AOs) to restore normal splicing by blocking the recognition of aberrant splice sites by the spliceosome represents an innovative means of potentially controlling certain inherited disorders affected by aberrant splicing. Selection of the appropriate target site is essential in the success of an AO therapy. In this study, in search for a splic...

The gastrin gene is expressed widely in pancreatic adenocarcinomas and the study aimed to assess its role in both the resistance of cancer cells to apoptosis and the sensitivity of cells to chemotherapeutic agents. Two human pancreatic cell lines, PAN1 and BXPC3, expressed gastrin at both the RNA and protein levels and are shown to be representative of human pancreatic adenocarcinomas in terms ...

The B cell-specific activator protein (BSAP) is a DNA-binding transcription factor expressed in pro-B, pre-B, and mature B cells, but not in plasma cells. In this study, we explored the role of BSAP in B cell function by assessing how the content of this protein varies in cells driven by proliferative stimuli and, conversely, how artificial manipulation of BSAP activity affects cell proliferati...

Plasmids are self-replicating extrachromosomal DNA molecules found in Gram-negative and Gram-positive bacteria as well as in some yeast and other fungi. Although most of them are covalently closed circular double-stranded DNA molecules, recently linear plasmids have been isolated from different bacteria. In general, plasmids are not essential for the survival of bacteria, but they may neverthel...